Evaluating the Role of Exogenously Applied Ascorbic Acid in Rescuing Soybean Plant Health in The Presence of Pathogen-Induced Oxidative Stress

Charcoal rot, caused by the soilborne hemibiotrophic fungus Macrophomina phaseolina, is a prevalent and economically significant plant disease. It is hypothesized that M. phaseolina induces oxidative stress-mediated senescence in plants. Infection by M. phaseolina results in the host’s accumulation...

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Bibliographic Details
Published in:Pathogens (Basel) 2022-09, Vol.11 (10), p.1117
Main Authors: Noor, Afsana, Little, Christopher R
Format: Article
Language:English
Subjects:
Accumulation
Apoptosis
Ascorbic acid
Care and treatment
Causes of
Cell cycle
Charcoal rot
Diseases and pests
Experiments
Fungal diseases of plants
H2O2
Hydrogen peroxide
Infections
Inoculation
Macrophomina phaseolina
Oxidation
Oxidative stress
Pathogens
Plant diseases
Reactive oxygen species
ROS scavenger
Scavenging
Senescence
Soybean
Soybeans
Testing
Variance analysis
Vitamin C
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Summary:Charcoal rot, caused by the soilborne hemibiotrophic fungus Macrophomina phaseolina, is a prevalent and economically significant plant disease. It is hypothesized that M. phaseolina induces oxidative stress-mediated senescence in plants. Infection by M. phaseolina results in the host’s accumulation of reactive oxygen species (ROS) that contribute toward basal defense. However, the production of ROS could also lead to cellular damage and senescence in host tissue. This study aimed to determine if ascorbic acid, a ROS scavenging molecule, could quench M. phaseolina-induced hydrogen peroxide (H2O2) generation in a soybean-M. phaseolina pathosystem. In vitro sensitivity tests showed that M. phaseolina isolates were sensitive to L-ascorbic acid (LAA) at concentrations of 10.5 to 14.3 mM based on IC50 (half-maximal inhibitory concentration) data. In planta cut-stem assays demonstrated that pre-treatment with 10 mM of either LAA (reduced form) or DHAA (dehydroascorbic acid; oxidized form) significantly decreased lesion length compared to the non-pretreated control and post-treatments with both ascorbic acid forms after M. phaseolina inoculation. Further, H2O2 quantification from ascorbic acid-pretreated tissue followed by M. phaseolina inoculation showed significantly less accumulation of H2O2 than the inoculated control or the mock-inoculated control. This result demonstrated that M. phaseolina not only induced H2O2 after host infection but also increased ROS-mediated senescence. This study shows the potential of ascorbic acid, an effective ROS scavenger, to limit ROS-mediated senescence associated with M. phaseolina infection.
ISSN:2076-0817
2076-0817
DOI:10.3390/pathogens11101117