A New Species of Scymnus (Coleoptera, Coccinellidae) from Pakistan with Mitochondrial Genome and Its Phylogenetic Implications

In this study, a new species of the subgenus belonging to the genus from Pakistan, sp. nov., was described and illustrated, with information on its distribution, host plants, and prey. Additionally, the completed mitochondrial genome (mitogenome) of the new species using high-throughput sequencing t...

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Bibliographic Details
Published in:Insects (Basel, Switzerland) Switzerland), 2024-05, Vol.15 (5), p.371
Main Authors: Iqbal, Zafar, Azad, Rashid, Chen, Xiao-Sheng, Lin, Xiao-Ling, Zhou, Zichen, Wang, Xing-Min, Nie, Rui-E
Format: Article
Language:English
Subjects:
Abdomen
Agriculture
Bayesian analysis
Coccinellidae
Coccinellinae
Coleoptera
Datasets
Entomology
Food and nutrition
Genes
Genetic aspects
Genomes
Host plants
Identification and classification
Ladybirds
Mitochondria
mitochondrial genome
New species
Next-generation sequencing
Nucleotide sequence
Phylogenetics
Phylogeny
Physiological aspects
Proteins
Pullus
Scymnini
Scymnus
Scymnus (Pullus)
Statistical inference
Trees
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Summary:In this study, a new species of the subgenus belonging to the genus from Pakistan, sp. nov., was described and illustrated, with information on its distribution, host plants, and prey. Additionally, the completed mitochondrial genome (mitogenome) of the new species using high-throughput sequencing technology was obtained. The genome contains the typical 37 genes (13 protein-coding genes, two ribosomal RNAs, and 22 transfer RNAs) and a non-coding control region, and is arranged in the same order as that of the putative ancestor of beetles. The AT content of the mitogenome is approximately 85.1%, with AT skew and GC skew of 0.05 and -0.43, respectively. The calculated values of relative synonymous codon usage (RSCU) determine that the codon UUA (L) has the highest frequency. Furthermore, we explored the phylogenetic relationship among 59 representatives of the Coccinellidae using Bayesian inference and maximum likelihood methods, the results of which strongly support the monophyly of Coccinellinae. The phylogenetic results positioned in a well-supported clade with and ( ) within the genus and the tribe Scymnini. The mitochondrial sequence of . ( .) will contribute to the mitochondrial genome database and provide helpful information for the identification and phylogeny of Coccinellidae.
ISSN:2075-4450
2075-4450
DOI:10.3390/insects15050371