Maternal Melatonin Therapy Attenuates Methyl-Donor Diet-Induced Programmed Hypertension in Male Adult Rat Offspring

• Published in: Nutrients 2018-10, Vol.10 (10), p.1407
• Main Authors: Tain, You-Lin, Chan, Julie Y H, Lee, Chien-Te, Hsu, Chien-Ning
• Format: Article
• Language: English
• Subjects: Adult Children; Animals; developmental origins of adult health and disease (DOHaD); Diet - adverse effects; Diet - methods; Female; hypertension; Hypertension - etiology; Hypertension - prevention & control; Male; Maternal Exposure - adverse effects; Maternal Nutritional Physiological Phenomena; melatonin; Melatonin - therapeutic use; methylation; nutrient-sensing signal; oxidative stress; Pregnancy; Prenatal Exposure Delayed Effects - etiology; Prenatal Exposure Delayed Effects - prevention & control; Rats; Rats, Sprague-Dawley
• ISSN: 2072-6643; 2072-6643
• DOI: 10.3390/nu10101407

Although pregnant women are advised to consume methyl-donor food, some reports suggest an adverse outcome. We investigated whether maternal melatonin therapy can prevent hypertension induced by a high methyl-donor diet. Female Sprague-Dawley rats received either a normal diet, a methyl-deficient diet (L-MD), or a high methyl-donor diet (H-MD) during gestation and lactation. Male offspring were assigned to four groups ( = 7⁻8/group): control, L-MD, H-MD, and H-MD rats were given melatonin (100 mg/L) with their drinking water throughout the period of pregnancy and lactation (H-MD+M). At 12 weeks of age, male offspring exposed to a L-MD or a H-MD diet developed programmed hypertension. Maternal melatonin therapy attenuated high methyl-donor diet-induced programmed hypertension. A maternal L-MD diet and H-MD diet caused respectively 938 and 806 renal transcripts to be modified in adult offspring. The protective effects of melatonin against programmed hypertension relate to reduced oxidative stress, increased urinary NO₂ level, and reduced renal expression of sodium transporters. A H-MD or L-MD diet may upset the balance of methylation status, leading to alterations of renal transcriptome and programmed hypertension. A better understanding of reprogramming effects of melatonin might aid in developing a therapeutic strategy for the prevention of hypertension in adult offspring exposed to an excessive maternal methyl-supplemented diet.