Influence of PAHs on ligninolytic enzymes of the fungus Pleurotus ostreatus D1

Polycyclic aromatic hydrocarbons (PAHs), their derivatives, and their degradation products were assayed for the ability to enhance activities of ligninolytic enzymes (laccase and versatile peroxidase) of the fungus Pleurotus ostreatus D1. The activities of both laccase and versatile peroxidase were...

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Bibliographic Details
Published in:Central European journal of biology 2010-02, Vol.5 (1), p.83-94
Main Authors: Pozdnyakova, Natalia N., Nikiforova, Svetlana V., Turkovskaya, Olga V.
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cell Biology
Degradation products
Enzymes
Human Genetics
Immunology
Induction
Laccase
Life Sciences
ligninolytic enzymes
Oxidation
PAHs
Peroxidase
Pleurotus ostreatus
Polycyclic aromatic hydrocarbons
Research Article
Versatile peroxidase
Xenobiotics
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Summary:Polycyclic aromatic hydrocarbons (PAHs), their derivatives, and their degradation products were assayed for the ability to enhance activities of ligninolytic enzymes (laccase and versatile peroxidase) of the fungus Pleurotus ostreatus D1. The activities of both laccase and versatile peroxidase were induced by the PAHs, their derivatives, and their degradation products. Laccase was produced mostly in the first 7–10 days, whereas the production of versatile peroxidase began after 5–7 days of cultivation. Non-denaturing PAGE showed the presence of additional forms of laccase and versatile peroxidase in the presence of the xenobiotics in the cultivation medium. The difference in the production time for these enzymes may reflect that laccases are involved in the first stages of PAHs degradation and that versatile peroxidase can be necessary for oxidation of some degradation products. This is the first report on versatile peroxidase induction by PAHs and their derivatives.
ISSN:1895-104X
2391-5412
1644-3632
2391-5412
DOI:10.2478/s11535-009-0075-4