Engineering of a Bacterial Biosensor for the Detection of Chlorate in Food

Chlorate can contaminate food due to the use of chlorinated water for processing or equipment disinfection. Chronic exposure to chlorate in food and drinking water is a potential health concern. The current methods for detecting chlorate in liquids and foods are expensive and not easily accessible t...

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Bibliographic Details
Published in:Biosensors (Basel) 2023-06, Vol.13 (6), p.629
Main Authors: Vergnes, Alexandra, Becam, Jérôme, Loiseau, Laurent, Ezraty, Benjamin
Format: Article
Language:English
Subjects:
Bacteria
bacteria biosensor
Bioassays
Biosensing Techniques
Biosensors
Chlorate
chlorate detection
Chlorates
Chronic exposure
Design and construction
Disinfection
Drinking water
E coli
Escherichia coli
Food
Food contamination
Genetic engineering
Glycerol
Growth conditions
Health aspects
Iodine
Life Sciences
Measurement
Methionine
methionine sulfoxide reductase
Nitrates
Potassium
Reductases
Scientific imaging
Statistical analysis
Testing
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Summary:Chlorate can contaminate food due to the use of chlorinated water for processing or equipment disinfection. Chronic exposure to chlorate in food and drinking water is a potential health concern. The current methods for detecting chlorate in liquids and foods are expensive and not easily accessible to all laboratories, highlighting an urgent need for a simple and cost-effective method. The discovery of the adaptation mechanism of to chlorate stress, which involves the production of the periplasmic Methionine Sulfoxide Reductase (MsrP), prompted us to use an strain with an fusion as a biosensor for detecting chlorate. Our study aimed to optimize the bacterial biosensor's sensitivity and efficiency to detect chlorate in various food samples using synthetic biology and adapted growth conditions. Our results demonstrate successful biosensor enhancement and provide proof of concept for detecting chlorate in food samples.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios13060629