A Graphene-Based Biosensing Platform Based on Regulated Release of an Aptameric DNA Biosensor

A novel biosensing platform was developed by integrating an aptamer-based DNA biosensor with graphene oxide (GO) for rapid and facile detection of adenosine triphosphate (ATP, as a model target). The DNA biosensor, which is locked by GO, is designed to contain two sensing modules that include recogn...

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Bibliographic Details
Published in:Sensors (Basel, Switzerland) Switzerland), 2015-11, Vol.15 (11), p.28244-28256
Main Authors: Mao, Yu, Chen, Yongli, Li, Song, Lin, Shuo, Jiang, Yuyang
Format: Article
Language:English
Subjects:
Adenosine triphosphate
Adsorption
aptamer
Aptamers, Nucleotide - chemistry
ATP
Biology
Biosensing Techniques - methods
Biosensors
Deoxyribonucleic acid
Design
Detection
DNA - analysis
DNA - chemistry
DNA biosensor
Enzymes
Graphene
graphene oxide
Graphite - chemistry
Isothermal amplification
Nanostructure
Platforms
Polymerization
Sensors
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Summary:A novel biosensing platform was developed by integrating an aptamer-based DNA biosensor with graphene oxide (GO) for rapid and facile detection of adenosine triphosphate (ATP, as a model target). The DNA biosensor, which is locked by GO, is designed to contain two sensing modules that include recognition site for ATP and self-replication track that yields the nicking domain for Nt.BbvCI. By taking advantage of the different binding affinity of single-stranded DNA, double-stranded DNA and aptamer-target complex toward GO, the DNA biosensor could be efficiently released from GO in the presence of target with the help of a complementary DNA strand (CPDNA) that partially hybridizes to the DNA biosensor. Then, the polymerization/nicking enzyme synergetic isothermal amplification could be triggered, leading to the synthesis of massive DNA amplicons, thus achieving an enhanced sensitivity with a wide linear dynamic response range of four orders of magnitude and good selectivity. This biosensing strategy expands the applications of GO-DNA nanobiointerfaces in biological sensing, showing great potential in fundamental research and biomedical diagnosis.
ISSN:1424-8220
1424-8220
DOI:10.3390/s151128244