Evaluation of the Microbial Profile on the Polydioxanone Membrane and the Collagen Membrane Exposed to Multi-Species Subgingival Biofilm: An In Vitro Study

Dehiscence in surgeries involving membranes often leads to bacterial contamination, hindering the healing process. This study assessed bacterial colonization on various membrane materials. Polydioxanone (PDO) membranes, with thicknesses of 0.5 mm and 1 mm, and a collagen membrane were examined. Pack...

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Published in:Membranes (Basel) 2023-12, Vol.13 (12), p.907
Main Authors: Cintra Moreira, Marcus Vinícius, Figueiredo, Luciene C, da Cunha Melo, Marcelo Augusto Ruiz, Uyeda, Fabio Hideaki, da Silva, Lucas Daylor Aguiar, Macedo, Tatiane Tiemi, Sacco, Roberto, Mourão, Carlos Fernando, Shibli, Jamil A, Bueno-Silva, Bruno
Format: Article
Language:English
Subjects:
Analysis
Bacteria
Biofilms
Campylobacter
Care and treatment
Collagen
collagen membrane
Dehiscence
Deoxyribonucleic acid
Dimethylpolysiloxane
DNA
Evaluation
Gums
Hybridization
Membranes
Membranes (Biology)
Microbial mats
microbial profile
Microorganisms
Oral health
Pathogens
Phosphatase
polydioxanone membrane
Polystyrene
Polystyrene resins
Properties
Sheep
Statistical analysis
subgingival biofilm
Tissue engineering
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container_end_page
container_issue 12
container_start_page 907
container_title Membranes (Basel)
container_volume 13
creator Cintra Moreira, Marcus Vinícius
Figueiredo, Luciene C
da Cunha Melo, Marcelo Augusto Ruiz
Uyeda, Fabio Hideaki
da Silva, Lucas Daylor Aguiar
Macedo, Tatiane Tiemi
Sacco, Roberto
Mourão, Carlos Fernando
Shibli, Jamil A
Bueno-Silva, Bruno
description Dehiscence in surgeries involving membranes often leads to bacterial contamination, hindering the healing process. This study assessed bacterial colonization on various membrane materials. Polydioxanone (PDO) membranes, with thicknesses of 0.5 mm and 1 mm, and a collagen membrane were examined. Packages containing polystyrene pins were crafted using these membranes, attached to 24-well plates, and exposed to oral bacteria from supra and subgingival biofilm. After a week's anaerobic incubation, biofilm formation was evaluated using the DNA-DNA hybridization test. Statistical analysis employed the Kruskal-Wallis test with Dunn's post hoc test. The biofilm on the polystyrene pins covered by the 0.5 mm PDO membrane showed a higher count of certain pathogens. The collagen membrane had a greater total biofilm count on its inner surface compared to both PDO membranes. The external collagen membrane face had a higher total biofilm count than the 0.5 mm PDO membrane. Furthermore, the 1 mm PDO membrane exhibited a greater count of specific pathogens than its 0.5 mm counterpart. In conclusion, the collagen membrane presented more biofilm and pathogens both internally and on its inner surface.
doi_str_mv 10.3390/membranes13120907
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This study assessed bacterial colonization on various membrane materials. Polydioxanone (PDO) membranes, with thicknesses of 0.5 mm and 1 mm, and a collagen membrane were examined. Packages containing polystyrene pins were crafted using these membranes, attached to 24-well plates, and exposed to oral bacteria from supra and subgingival biofilm. After a week's anaerobic incubation, biofilm formation was evaluated using the DNA-DNA hybridization test. Statistical analysis employed the Kruskal-Wallis test with Dunn's post hoc test. The biofilm on the polystyrene pins covered by the 0.5 mm PDO membrane showed a higher count of certain pathogens. The collagen membrane had a greater total biofilm count on its inner surface compared to both PDO membranes. The external collagen membrane face had a higher total biofilm count than the 0.5 mm PDO membrane. Furthermore, the 1 mm PDO membrane exhibited a greater count of specific pathogens than its 0.5 mm counterpart. 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subjects Analysis
Bacteria
Biofilms
Campylobacter
Care and treatment
Collagen
collagen membrane
Dehiscence
Deoxyribonucleic acid
Dimethylpolysiloxane
DNA
Evaluation
Gums
Hybridization
Membranes
Membranes (Biology)
Microbial mats
microbial profile
Microorganisms
Oral health
Pathogens
Phosphatase
polydioxanone membrane
Polystyrene
Polystyrene resins
Properties
Sheep
Statistical analysis
subgingival biofilm
Tissue engineering
title Evaluation of the Microbial Profile on the Polydioxanone Membrane and the Collagen Membrane Exposed to Multi-Species Subgingival Biofilm: An In Vitro Study
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