Nutrient availability influences E. coli biofilm properties and the structure of purified curli amyloid fibers

Bacterial biofilms are highly adaptable and resilient to challenges. Nutrient availability can induce changes in biofilm growth, architecture and mechanical properties. Their extracellular matrix plays an important role in achieving biofilm stability under different environmental conditions. Curli a...

Full description

Saved in:
Bibliographic Details
Published in:NPJ biofilms and microbiomes 2024-12, Vol.10 (1), p.143-12, Article 143
Main Authors: Siri, Macarena, Vázquez-Dávila, Mónica, Sotelo Guzman, Carolina, Bidan, Cécile M.
Format: Article
Language:English
Subjects:
631/1647
631/326/46
Amyloid - chemistry
Amyloid - metabolism
Bacteria
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biofilms
Biofilms - growth & development
Biomedical and Life Sciences
E coli
Environmental conditions
Escherichia coli - physiology
Escherichia coli Proteins - metabolism
Extracellular matrix
Fibers
Influence
Life Sciences
Mechanical properties
Medical Microbiology
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
Morphology
Nutrient availability
Nutrients
Nutrients - metabolism
Spectrum analysis
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Bacterial biofilms are highly adaptable and resilient to challenges. Nutrient availability can induce changes in biofilm growth, architecture and mechanical properties. Their extracellular matrix plays an important role in achieving biofilm stability under different environmental conditions. Curli amyloid fibers are critical for the architecture and stiffness of E. coli biofilms, but how this major matrix component adapts to different environmental cues remains unclear. We investigated, for the first time, the effect of nutrient availability both on biofilm material properties and on the structure and properties of curli amyloid fibers extracted from similar biofilms. Our results show that biofilms grown on low nutrient substrates are stiffer, contain more curli fibers, and these fibers present higher β-sheet content and chemical stability. Our multiscale study sheds new light on the relationship between bacterial matrix molecular structure and biofilm macroscopic properties. This knowledge will benefit the development of both anti-biofilm strategies and biofilm-based materials.
ISSN:2055-5008
2055-5008
DOI:10.1038/s41522-024-00619-0