TrkB-expressing paraventricular hypothalamic neurons suppress appetite through multiple neurocircuits

• Published in: Nature communications 2020-04, Vol.11 (1), p.1729-1729, Article 1729
• Main Authors: An, Juan Ji, Kinney, Clint E., Tan, Ji-Wei, Liao, Guey-Ying, Kremer, Eric J., Xu, Baoji
• Format: Article
• Language: English
• Subjects: 14/19; 38/1; 38/32; 45/41; 631/378/1488/1562; 631/378/340; 631/443/319/1557; 64/60; Activation; Animals; Appetite; Appetite - genetics; Brain-derived neurotrophic factor; Circuits; Deletion; Energy balance; Feeding Behavior - physiology; Female; Food intake; Humanities and Social Sciences; Hyperphagia; Hyperphagia - genetics; Hyperphagia - metabolism; Hypothalamus; Hypothalamus (lateral); Hypothalamus (ventromedial); Membrane Glycoproteins - genetics; Membrane Glycoproteins - metabolism; Mice; Mice, Inbred C57BL; Mice, Transgenic; multidisciplinary; Mutation; Neural networks; Neuromodulation; Neurons; Neurons - metabolism; Obesity; Obesity - genetics; Obesity - metabolism; Parabrachial nucleus; Parabrachial Nucleus - cytology; Parabrachial Nucleus - metabolism; Parabrachial Nucleus - physiopathology; Paraventricular Hypothalamic Nucleus - cytology; Paraventricular Hypothalamic Nucleus - metabolism; Protein-Tyrosine Kinases - genetics; Protein-Tyrosine Kinases - metabolism; Receptors; Science; Science (multidisciplinary); Substrates; TrkB receptors; Ventromedial Hypothalamic Nucleus - cytology; Ventromedial Hypothalamic Nucleus - metabolism
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-020-15537-w

The TrkB receptor is critical for the control of energy balance, as mutations in its gene ( NTRK2 ) lead to hyperphagia and severe obesity. The main neural substrate mediating the appetite-suppressing activity of TrkB, however, remains unknown. Here, we demonstrate that selective Ntrk2 deletion within paraventricular hypothalamus (PVH) leads to severe hyperphagic obesity. Furthermore, chemogenetic activation or inhibition of TrkB-expressing PVH (PVH TrkB ) neurons suppresses or increases food intake, respectively. PVH TrkB neurons project to multiple brain regions, including ventromedial hypothalamus (VMH) and lateral parabrachial nucleus (LPBN). We find that PVH TrkB neurons projecting to LPBN are distinct from those to VMH, yet Ntrk2 deletion in PVH neurons projecting to either VMH or LPBN results in hyperphagia and obesity. Additionally, TrkB activation with BDNF increases firing of these PVH neurons. Therefore, TrkB signaling is a key regulator of a previously uncharacterized neuronal population within the PVH that impinges upon multiple circuits to govern appetite. The TrkB receptor is known to regulate obesity via appetite control, but the underlying neural circuits are not known. Here, the authors show that selective modulation of TrkB+ neurons in the paraventricular hypothalamus regulates food intake via circuits to ventromedial hypothalamus and lateral parabrachial nucleus.