Eicosapentaenoic Acid Regulates Inflammatory Pathways through Modulation of Transcripts and miRNA in Adipose Tissue of Obese Mice

This study aims to investigate the global profiling of genes and miRNAs expression to explore the regulatory effects of eicosapentaenoic acid (EPA) in visceral adipose tissue (VAT) of obese mice. We used male mice, fed either a high-fat diet (HF) or HF supplemented with EPA (HF-EPA), for 11 weeks. R...

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Bibliographic Details
Published in:Biomolecules (Basel, Switzerland) Switzerland), 2020-09, Vol.10 (9), p.1292
Main Authors: Ramalho, Theresa, Pahlavani, Mandana, Kalupahana, Nishan, Wijayatunga, Nadeeja, Ramalingam, Latha, Jancar, Sonia, Moustaid-Moussa, Naima
Format: Article
Language:English
Subjects:
Adipose tissue
Archives & records
Body fat
CCR5 protein
Cell death
Chemotaxis
Diet
Eicosanoic acid
Eicosapentaenoic acid
Gene expression
Genetic aspects
Genomes
Genomics
Health aspects
High fat diet
Inflammation
Insulin
leukotriene-B4
Metabolism
Metabolites
MicroRNA
MicroRNAs
miRNA
Obesity
Polymerase chain reaction
Quality control
Software
Tumor necrosis factor-TNF
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Summary:This study aims to investigate the global profiling of genes and miRNAs expression to explore the regulatory effects of eicosapentaenoic acid (EPA) in visceral adipose tissue (VAT) of obese mice. We used male mice, fed either a high-fat diet (HF) or HF supplemented with EPA (HF-EPA), for 11 weeks. RNA, and small RNA profiling, were performed by RNAseq analysis. We conducted analyses using Ingenuity Pathway Analysis software (IPA ) and validated candidate genes and miRNAs related to lipid mediators and inflammatory pathways using qRT-PCR. We identified 153 genes differentially downregulated, and 62 microRNAs differentially expressed in VAT from HF-EPA compared to HF. Genes with a positive association with inflammation, chemotaxis, insulin resistance, and inflammatory cell death, such as , , , , , , and , were downregulated by EPA. Moreover, EPA significantly reduced LTB4 levels, a lipid mediator with a central role in inflammation and insulin resistance in obesity. The pathways and mRNA/microRNA interactions identified in our study corroborated with data validated for inflammatory genes and miRNAs. Together, our results identified key VAT inflammatory targets and pathways, which are regulated by EPA. These targets merit further investigation to better understand the protective mechanisms of EPA in obesity-associated inflammation.
ISSN:2218-273X
2218-273X
DOI:10.3390/biom10091292