Heterologous 1,3-Propanediol Production Using Different Recombinant Clostridium beijerinckii DSM 6423 Strains

1,3-propanediol (1,3-PDO) is a valuable basic chemical, especially in the polymer industry to produce polytrimethylene terephthalate. Unfortunately, the production of 1,3-PDO mainly depends on petroleum products as precursors. Furthermore, the chemical routes have significant disadvantages, such as...

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Bibliographic Details
Published in:Microorganisms (Basel) 2023-03, Vol.11 (3), p.784
Main Authors: Schoch, Teresa, Baur, Tina, Kunz, Johanna, Stöferle, Sophia, Dürre, Peter
Format: Article
Language:English
Subjects:
1,3-Propanediol
1,3-propanediol production
Antibiotics
Biodiesel fuels
Cloning
Clostridium
Clostridium beijerinckii
Clostridium diolis
Clostridium pasteurianum
Comparative analysis
Dehydrogenases
E coli
Enzymes
Fermentation
Genes
Genetic aspects
Genetic engineering
Genomes
Glycerol
glycerol fermentation
Glycols
Growth conditions
Measurement
Microorganisms
Petroleum products
Physiological aspects
Plasmids
Polymers
promoter effects
Trace elements
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Summary:1,3-propanediol (1,3-PDO) is a valuable basic chemical, especially in the polymer industry to produce polytrimethylene terephthalate. Unfortunately, the production of 1,3-PDO mainly depends on petroleum products as precursors. Furthermore, the chemical routes have significant disadvantages, such as environmental issues. An alternative is the biobased fermentation of 1,3-PDO from cheap glycerol. DSM 6423 was originally reported to produce 1,3-PDO. However, this could not be confirmed, and a genome analysis revealed the loss of an essential gene. Thus, 1,3-PDO production was genetically reinstalled. Genes for 1,3-PDO production from DSM 525 and DSM 15410 (formerly ) were introduced into DSM 6423 to enable 1,3-PDO production from glycerol. 1,3-PDO production by recombinant strains were investigated under different growth conditions. 1,3-PDO production was only observed for [pMTL83251_P _1,3-PDO.diolis], which harbors the genes of DSM 15410. By buffering the growth medium, production could be increased by 74%. Furthermore, the effect of four different promoters was analyzed. The use of the constitutive promoter from led to a 167% increase in 1,3-PDO production compared to the initial recombinant approach.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms11030784