MiR-107 function as a tumor suppressor gene in colorectal cancer by targeting transferrin receptor 1

While microRNAs (miRNAs) are known to play a critical role in the progression of colorectal cancer, the role of miR-107 remains unknown. We evaluated its role and explored the underlying mechanism. MTT, wound-healing, transwell migration and transwell invasion assays were performed to evaluate the r...

Full description

Saved in:
Bibliographic Details
Published in:Cellular & molecular biology letters 2019-05, Vol.24 (1), p.31-31, Article 31
Main Authors: Fu, Yuxiang, Lin, Liewen, Xia, Ligang
Format: Article
Language:English
Subjects:
Base Sequence
Biomarkers
Cancer
Cancer progress
Cell cycle
Cell growth
Cell Line, Tumor
Cell migration
Cell Movement - genetics
Cell proliferation
Cell Proliferation - genetics
Chemotherapy
Cloning
Colorectal cancer
Colorectal carcinoma
Colorectal Neoplasms - genetics
Colorectal Neoplasms - pathology
Down-Regulation
Gene Expression Regulation, Neoplastic
Genes
Genes, Tumor Suppressor
Humans
Medical prognosis
Metastases
Metastasis
microRNA107 (miR-107)
MicroRNAs
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA
Neoplasm Invasiveness
Reagents
Receptors, Transferrin - metabolism
Reporter gene
Research Letter
Transferrin receptor 1 (TFR1)
Transferrins
Tumor cell lines
Tumor suppressor genes
Up-Regulation - genetics
Western blotting
Wound healing
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:While microRNAs (miRNAs) are known to play a critical role in the progression of colorectal cancer, the role of miR-107 remains unknown. We evaluated its role and explored the underlying mechanism. MTT, wound-healing, transwell migration and transwell invasion assays were performed to evaluate the role of miR-107 in SW629 cell proliferation, migration and invasion. Real time-PCR and dual-luciferase reporter gene, TFR1 overexpression and western blotting assays were used to explore the underlying mechanism. MiR-107 is downregulated in colorectal cancer tissues and several human colorectal cancer cell lines. Low miR-107 expression often indicates a poor survival rate for colorectal cancer patients. MiR-107 suppresses the proliferation, migration and invasion of SW620 cells by negatively regulating transferrin receptor 1 (TFR1). MiR-107 suppresses the metastasis of colorectal cancer and could be a potential therapy target in colorectal cancer patients.
ISSN:1425-8153
1689-1392
DOI:10.1186/s11658-019-0155-z