Immature Seed Endosperm and Embryo Proteomics of the Lotus ( Nelumbo Nucifera Gaertn.) by One-Dimensional Gel-Based Tandem Mass Spectrometry and a Comparison with the Mature Endosperm Proteome

Lotus ( Gaertn.) seed proteome has been the focus of our studies, and we have recently established the first proteome dataset for its mature seed endosperm. The current study unravels the immature endosperm, as well as the embryo proteome, to provide a comprehensive dataset of the lotus seed protein...

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Bibliographic Details
Published in:Proteomes 2015-08, Vol.3 (3), p.184-235
Main Authors: Moro, Carlo F, Fukao, Yoichiro, Shibato, Junko, Rakwal, Randeep, Agrawal, Ganesh Kumar, Shioda, Seiji, Kouzuma, Yoshiaki, Yonekura, Masami
Format: Article
Language:English
Subjects:
1-DGE
LC-MS/MS
lotus
plant proteomics
proteome analysis
seed
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Summary:Lotus ( Gaertn.) seed proteome has been the focus of our studies, and we have recently established the first proteome dataset for its mature seed endosperm. The current study unravels the immature endosperm, as well as the embryo proteome, to provide a comprehensive dataset of the lotus seed proteins and a comparison between the mature and immature endosperm tissues across the seed's development. One-dimensional gel electrophoresis (SDS-PAGE) linked with tandem mass spectrometry provided a protein inventory of the immature endosperm (122 non-redundant proteins) and embryo (141 non-redundant proteins) tissues. Comparing with the previous mature endosperm dataset (66 non-redundant proteins), a total of 206 non-redundant proteins were identified across all three tissues of the lotus seed. Results revealed some significant differences in proteome composition between the three lotus seed tissues, most notably between the mature endosperm and its immature developmental stage shifting the proteins from nutrient production to nutrient storage.
ISSN:2227-7382
2227-7382
DOI:10.3390/proteomes3030184