Bee Venom Effect on Glioblastoma Cells Viability and Gelatinase Secretion

The involvement of MMP-2 and MMP-9 in the pathogenesis of various kinds of cancers including glioblastoma is well documented. The evaluation of the anticancer potential of honey bee ( ) venom (BV) consisting of the inhibition of MMP-2 and MMP-9 secretion in a glioblastoma cell culture model was the...

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Bibliographic Details
Published in:Frontiers in neuroscience 2022-02, Vol.16, p.792970-792970
Main Authors: Małek, Agata, Kocot, Joanna, Mitrowska, Kamila, Posyniak, Andrzej, Kurzepa, Jacek
Format: Article
Language:English
Subjects:
anticancer potential
Apis mellifera
bee venom
Brain cancer
Brain research
Cancer therapies
Cell culture
Cell growth
Cell lines
Cell viability
Chemotherapy
Colorimetry
Cytotoxicity
Gelatin
Gelatinase
Gelatinase A
Gelatinase B
Glioblastoma
Glioblastoma cells
Glioma
Hippocampus
Metastasis
MMP-2
MMP-9
Neuroscience
Secretion
Statistical analysis
Tumors
Venom
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Summary:The involvement of MMP-2 and MMP-9 in the pathogenesis of various kinds of cancers including glioblastoma is well documented. The evaluation of the anticancer potential of honey bee ( ) venom (BV) consisting of the inhibition of MMP-2 and MMP-9 secretion in a glioblastoma cell culture model was the aim of the study. 8-MG-BA and GAMG human primary glioblastoma cell lines vs. HT-22 mouse hippocampal neuronal cells were applied for the study. The BV dose (0.5, 1.0, 1.25, 1.5, 1.75, 2.0, 2.5, and 5.0 μg/ml) and time-dependent (24, 48, 72 h) cytotoxicity was evaluated with the tetrazolium-based colorimetric assay (MTT test). MMP-2 and MMP-9 activities in the cell culture medium under different BV concentrations were determined by gelatin zymography. A dose and time-dependent BV effect on cytotoxicity of both glioblastoma cell lines and hippocampus line was observed. The weakest, but statistically important effect was exerted by BV on HT-22 cells. The greatest cytotoxic effect of BV was observed on the 8-MG-BA line, where a statistically significant reduction in viability was observed at the lowest BV dose and the shortest incubation time. The reduction of both gelatinases secretion was observed at 8-MG-BA and GAMG lines without significant effect of HT-22 cell line. studies indicate that BV has both cytotoxic and inhibitory effects on the secretion of MMP-2 and MMP-9 in selected lines of glioma, suggesting anticancer properties of BV.
ISSN:1662-4548
1662-453X
1662-453X
DOI:10.3389/fnins.2022.792970