DRL1 , Encoding A NAC Transcription Factor, Is Involved in Leaf Senescence in Grapevine

The NAC (for NAM, ATAF1,2, and CUC2) proteins family are plant-specific transcription factors, which play important roles in leaf development and response to environmental stresses. In this study, an NAC gene, , isolated from grapevine L. "Yatomi Rose", was shown to be involved in leaf sen...

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Published in:International journal of molecular sciences 2019-05, Vol.20 (11), p.2678
Main Authors: Zhu, Ziguo, Li, Guirong, Yan, Chaohui, Liu, Li, Zhang, Qingtian, Han, Zhen, Li, Bo
Format: Article
Language:English
Subjects:
ABA
Abscisic acid
Biosynthesis
Chlorophyll
Dioxygenase
Gene expression
Genotype & phenotype
Grapevine
Jasmonic acid
leaf senescence
Leaves
NAC transcription factor
Photosystem II
Physiology
Proteins
Quantum efficiency
Salicylic acid
Seeds
Senescence
Sensitivity analysis
Signal transduction
Tobacco
Transcription factors
Transgenic plants
Zeaxanthin
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Summary:The NAC (for NAM, ATAF1,2, and CUC2) proteins family are plant-specific transcription factors, which play important roles in leaf development and response to environmental stresses. In this study, an NAC gene, , isolated from grapevine L. "Yatomi Rose", was shown to be involved in leaf senescence. The quantity of transcripts decreased with advancing leaf senescence in grapevine. Overexpressing the gene in tobacco plants significantly delayed leaf senescence with respect to chlorophyll concentration, potential quantum efficiency of photosystem II (Fv/Fm), and ion leakage. Moreover, exogenous abscisic acid (ABA) markedly reduced the expression of , and the ABA and salicylic acid (SA) concentration was lower in the -overexpressing transgenic plants than in the wild-type plants. The transgenic plants exhibited reduced sensitivity to ABA-induced senescence but no significant change in the sensitivity to jasmonic acid-, SA- or ethylene-induced senescence. Transcriptomic analysis and RNA expression studies also indicated that the transcript abundance of genes associated with ABA biosynthesis and regulation, including 9- -epoxycarotenoid dioxygenase ( ), , zeaxanthin epoxidase1 ( ), ABA DEFICIENT2 ( ), , and ABA INSENSITIVE 2 ( ), was markedly reduced in the -overexpressing plants. These results suggested that plays a role as a negative regulator of leaf senescence by regulating ABA synthesis.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms20112678