Bioremediation of Azo Dye Brown 703 by Pseudomonas aeruginosa: An Effective Treatment Technique for Dye-Polluted Wastewater

• Published in: Microbiology research 2023-09, Vol.14 (3), p.1049-1066
• Main Authors: Ullah Khan, Asad, Zahoor, Muhammad, Ur Rehman, Mujaddad, Ikram, Muhammad, Zhu, Daochen, Naveed Umar, Muhammad, Ullah, Riaz, Ali, Essam A.
• Format: Article
• Language: English
• Subjects: Azo dyes; Bacteria; Biodegradation; bioreactor; Bioremediation; Carcinogens; Decoloring; Decolorization; Degradation; Dietary supplements; Dumping; Dyes; Health risks; Industrial development; Infrared analysis; metabolites; Optimization; Pollutants; Pseudomonas aeruginosa; Strains (organisms); Temperature effects; textile effluents; Textile industry; Textile industry wastes; Textile industry wastewaters; Wastewater pollution; Wastewater treatment; water pollution
• ISSN: 2036-7481; 2036-7481
• DOI: 10.3390/microbiolres14030070

Dye-polluted wastewater poses a serious threat to humans’, animals’ and plants’ health, and to avoid these health risks in the future, the treatment of wastewater containing dyes is necessary before its release to environment. Herein, a biological approach is used; the textile azo dye brown 703 is degraded utilizing Pseudomonas aeruginosa. The bacterial strain was isolated from textile wastewater dumping sites in Mingora, Swat. The optimization for bacterial degradation was carried out on the nutrient broth medium, which was then subjected to a variety of environmental physicochemical conditions and nutritional source supplementation before being tested. Under micro-aerophilic circumstances, the maximum decolorization and degradation of dye occurred at a 20 ppm dye concentration within 3 days of incubation at a neutral pH and 38 °C. The decrease in the intensity of the absorbance peak in the UV–Vis spectrum was used to measure the extent of decolorization. Initially, 15 bacterial strains were isolated from the textile effluent. Out of these strains, Pseudomonas aeruginosa was found to be the most potent degrading bacteria, with a degradation extent of around 71.36% at optimum conditions. The appearance and disappearance of some new peaks in the FT-IR analysis after the degradation of brown 703 showed that the dye was degraded by Pseudomonas aeruginosa. The GC–MS analysis performed helped in identifying the degraded compounds of azo dye that were utilized in illustrating the under-study process of brown 703 degradation. The biodegradation brought about by Pseudomonas aeruginosa can be employed successfully in the future as an eco-friendly approach with far reaching results.