A Novel Multi-Component Formulation Reduces Inflammation In Vitro and Clinically Lessens the Symptoms of Chronic Eczematous Skin

• Published in: International journal of molecular sciences 2023-08, Vol.24 (16), p.12979
• Main Authors: Kim, Jihee, Jung, Eunjoong, Yang, Wonmi, Kim, Chun-Kang, Durnaoglu, Serpen, Oh, In-Rok, Kim, Chan-Wha, Sinskey, Anthony J., Mihm, Martin C., Lee, Ju Hee
• Format: Article
• Language: English
• Subjects: 3D skin model; Analysis; anti-inflammation; Atopic dermatitis; Care and treatment; Cell culture; Chloride; chronic eczema; cytokine; Cytokines; Dermatitis; Erythema; Fibroblasts; Inflammation; keratinocytes; Skin; Sodium; Steroids; Tumor necrosis factor; Tumor necrosis factor-TNF
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms241612979

Long-term treatments for inflammatory skin diseases like atopic dermatitis or eczema can cause adverse effects. Super Protein Multifunction (SPM) was investigated as a potential treatment for managing skin inflammation by monitoring the expression of pro-inflammatory cytokines induced using LPS and poly(I:C)/TNFα in HaCaT keratinocytes and Hs27 fibroblasts as measured via RT-PCR. SPM solution was also assessed for its effect on cytokine release, measured using ELISA, in a UVB-irradiated 3D human skin model. To evaluate the efficiency of SPM, 20 patients with mild eczematous skin were randomized to receive SPM or vehicle twice a day for three weeks in a double-blind controlled trial. In vitro studies showed SPM inhibited inflammation-induced IL-1β, IL-6, IL-33, IL-1α, TSLP, and TNFα expression or release. In the clinical study, the SPM group showed significant improvements in the IGA, PA, and DLQI scores compared to the vehicle group. Neither group showed significant differences in VAS (pruritus). Histological analysis showed reduced stratum corneum thickness and inflammatory cell infiltration. The results suggest that SPM may reduce inflammation in individuals with chronic eczematous skin.