Modulation of CAT-2B-Mediated l-Arginine Uptake and Nitric Oxide Biosynthesis in HCT116 Cell Line Through Biological Activity of 4'-Geranyloxyferulic Acid Extract from Quinoa Seeds

• Published in: International journal of molecular sciences 2019-07, Vol.20 (13), p.3262
• Main Authors: Franceschelli, Sara, Gatta, Daniela Maria Pia, Pesce, Mirko, Ferrone, Alessio, Quiles, José Luis, Genovese, Salvatore, Epifano, Francesco, Fiorito, Serena, Taddeo, Vito Alessandro, Patruno, Antonia, Grilli, Alfredo, Felaco, Mario, Speranza, Lorenza
• Format: Article
• Language: English
• Subjects: Angiogenesis; Anti-inflammatory agents; Anticarcinogenic Agents - chemistry; Anticarcinogenic Agents - pharmacology; Antioxidants; Arginine; Arginine - metabolism; Biological activity; Biosynthesis; CAT-2B; Catalytic activity; Cationic Amino Acid Transporter 2 - metabolism; Cell growth; Chemokines; Chenopodium quinoa - chemistry; Colorectal cancer; Coumaric Acids - chemistry; Coumaric Acids - pharmacology; Enzymes; Gene expression; HCT116 Cells; Humans; IL-6; Inflammation; Inflammation - metabolism; Inflammation - prevention & control; l -arginine; Metabolites; Mucosa; Neoplasms - metabolism; Neoplasms - prevention & control; Nitric oxide; Nitric Oxide - metabolism; Nitric-oxide synthase; Nitrotyrosine; Oxidative stress; Oxidative Stress - drug effects; Phytochemicals; Quinoa; Retention; Seeds; Seeds - chemistry; Superoxide; TNF-α; Tumor necrosis factor
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20133262

is a "pseudocereal" grain which attracts a lot of attention in the scientific community as it has a positive effect on health. Here, we investigate the presence of biologically active O-prenylated phenylpropanoids in the ethanol extract of commercially available quinoa seeds. We claim that 4'-Geranyloxyferulic acid (GOFA) was the only phytochemical product found that belongs to quinoa's group secondary metabolites. We studied the changes in the oxidative and inflammatory status of the cellular environment in HCT 116 cell line processed with quinoa extract and its component GOFA; the implementation was done through the analysis of the antioxidant enzymes (SOD and CAT), the pro-inflammatory components (iNOS, IL-6 and TNF-α), and the products of intermediary metabolism (ONOO , O ). Moreover, the l-arginine uptake was proposed as a target of the tested compounds. We demonstrated that the GOFA, through a decrease of the CAT-2B expression, leads to a reduction of the l-arginine uptake, downregulating the harmful iNOS and restoring the altered redox state. These results propose a new molecular target involved in the reduction of the critical inflammatory process responsible for the cancer progression.