Genetic Analysis and QTL Detection on Fiber Traits Using Two Recombinant Inbred Lines and Their Backcross Populations in Upland Cotton

• Published in: G3 : genes - genomes - genetics 2016-09, Vol.6 (9), p.2717-2724
• Main Authors: Shang, Lianguang, Wang, Yumei, Wang, Xiaocui, Liu, Fang, Abduweli, Abdugheni, Cai, Shihu, Li, Yuhua, Ma, Lingling, Wang, Kunbo, Hua, Jinping
• Format: Article
• Language: English
• Subjects: backcross population; Chromosome Mapping; Cotton Fiber; Crosses, Genetic; Environment; fiber quality; Genetic Linkage; Gossypium - genetics; Gossypium - growth & development; Investigations; Phenotype; QTL; Quantitative Trait Loci - genetics; recombinant inbred line; Upland cotton
• ISSN: 2160-1836; 2160-1836
• DOI: 10.1534/g3.116.031302

Abstract Cotton fiber, a raw natural fiber material, is widely used in the textile industry. Understanding the genetic mechanism of fiber traits is helpful for fiber quality improvement. In the present study, the genetic basis of fiber quality traits was explored using two recombinant inbred lines (RILs) and corresponding backcross (BC) populations under multiple environments in Upland cotton based on marker analysis. In backcross populations, no significant correlation was observed between marker heterozygosity and fiber quality performance and it suggested that heterozygosity was not always necessarily advantageous for the high fiber quality. In two hybrids, 111 quantitative trait loci (QTL) for fiber quality were detected using composite interval mapping, in which 62 new stable QTL were simultaneously identified in more than one environment or population. QTL detected at the single-locus level mainly showed additive effect. In addition, a total of 286 digenic interactions (E-QTL) and their environmental interactions [QTL × environment interactions (QEs)] were detected for fiber quality traits by inclusive composite interval mapping. QE effects should be considered in molecular marker-assisted selection breeding. On average, the E-QTL explained a larger proportion of the phenotypic variation than the main-effect QTL did. It is concluded that the additive effect of single-locus and epistasis with few detectable main effects play an important role in controlling fiber quality traits in Upland cotton.