Toward DNA-Based Recording of Biological Processes

Exploiting the inherent compatibility of DNA-based data storage with living cells, various cellular recording approaches have been developed for recording and retrieving biologically relevant signals in otherwise inaccessible locations, such as inside the body. This review provides an overview of th...

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Bibliographic Details
Published in:International journal of molecular sciences 2024-09, Vol.25 (17), p.9233
Main Authors: Jang, Hyeri, Yim, Sung Sun
Format: Article
Language:English
Subjects:
Animals
Arrays
Bar codes
cellular memory
CRISPR
CRISPR-Cas Systems
DNA - genetics
DNA Methylation
DNA-based data storage
Gene expression
Genome editing
Humans
molecular recording
Mutation
synthetic biology
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Summary:Exploiting the inherent compatibility of DNA-based data storage with living cells, various cellular recording approaches have been developed for recording and retrieving biologically relevant signals in otherwise inaccessible locations, such as inside the body. This review provides an overview of the current state of engineered cellular memory systems, highlighting their design principles, advantages, and limitations. We examine various technologies, including CRISPR-Cas systems, recombinases, retrons, and DNA methylation, that enable these recording systems. Additionally, we discuss potential strategies for improving recording accuracy, scalability, and durability to address current limitations in the field. This emerging modality of biological measurement will be key to gaining novel insights into diverse biological processes and fostering the development of various biotechnological applications, from environmental sensing to disease monitoring and beyond.
ISSN:1661-6596
1422-0067
1422-0067
DOI:10.3390/ijms25179233