Molecular and anatomical organization of the dorsal raphe nucleus

The dorsal raphe nucleus (DRN) is an important source of neuromodulators and has been implicated in a wide variety of behavioral and neurological disorders. The DRN is subdivided into distinct anatomical subregions comprised of multiple cell types, and its complex cellular organization has impeded e...

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Bibliographic Details
Published in:eLife 2019-08, Vol.8
Main Authors: Huang, Kee Wui, Ochandarena, Nicole E, Philson, Adrienne C, Hyun, Minsuk, Birnbaum, Jaclyn E, Cicconet, Marcelo, Sabatini, Bernardo L
Format: Article
Language:English
Subjects:
Animals
Basal ganglia
Correlation analysis
Dorsal raphe nucleus
Dorsal Raphe Nucleus - anatomy & histology
Dorsal Raphe Nucleus - cytology
Gene expression
Gene Expression Profiling
Genotype
Hybridization
In Situ Hybridization
Mice
Nervous system diseases
Neuroanatomical Tract-Tracing Techniques
Neurological diseases
Neuromodulation
Neurons
Neurons - classification
Neuroscience
Neurosciences
Phenotype
Physiological aspects
Raphe nuclei
Ribonucleic acid
RNA
RNA sequencing
Sequence Analysis, RNA
Serotonin
single cell RNAseq
Spatial Analysis
Surgery
Transcription
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Summary:The dorsal raphe nucleus (DRN) is an important source of neuromodulators and has been implicated in a wide variety of behavioral and neurological disorders. The DRN is subdivided into distinct anatomical subregions comprised of multiple cell types, and its complex cellular organization has impeded efforts to investigate the distinct circuit and behavioral functions of its subdomains. Here we used single-cell RNA sequencing, in situ hybridization, anatomical tracing, and spatial correlation analysis to map the transcriptional and spatial profiles of cells from the mouse DRN. Our analysis of 39,411 single-cell transcriptomes revealed at least 18 distinct neuron subtypes and 5 serotonergic neuron subtypes with distinct molecular and anatomical properties, including a serotonergic neuron subtype that preferentially innervates the basal ganglia. Our study lays out the molecular organization of distinct serotonergic and non-serotonergic subsystems, and will facilitate the design of strategies for further dissection of the DRN and its diverse functions.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.46464