Induction of apoptosis and role of paclitaxel-loaded hyaluronic acid-crosslinked nanoparticles in the regulation of AKT and RhoA

Cancer is a complex multifactorial disease and leading causes of death worldwide. Despite the development of many anticancer drugs, there is a reduced survival rate due to severe side effects. The nontargeted approach of convention drugs is one of the leading players in context to toxicity. Hyaluron...

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Bibliographic Details
Published in:Journal of advanced pharmaceutical technology and research 2020-07, Vol.11 (3), p.101-106
Main Authors: Chaudhry, Gul-e-Saba, Akim, Abdah, Zafar, Muhammad, Abdullah, M, Sung, Yeong, Muhammad, Tengku
Format: Article
Language:English
Subjects:
Adenosine diphosphate
Adenosine triphosphate
akt
AKT protein
Analysis
Antineoplastic drugs
Antitumor agents
Apoptosis
Biopolymers
Cancer
Cancer treatment
CD44 antigen
Cell activation
Cell death
Cell growth
Cell migration
Cell survival
drug delivery
Drug development
Flow cytometry
hyaluronan
Hyaluronic acid
Metastases
Nanoparticles
Original
p53 Protein
Paclitaxel
rho a
RhoA protein
Toxicity
Tumor cell lines
Tumor suppressor genes
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Summary:Cancer is a complex multifactorial disease and leading causes of death worldwide. Despite the development of many anticancer drugs, there is a reduced survival rate due to severe side effects. The nontargeted approach of convention drugs is one of the leading players in context to toxicity. Hyaluronan is a versatile bio-polymer and ligand of the receptor (CD44) on cancer cells. The MCF-7 and HT-29 cancer cell lines treated with hyaluronic acid-paclitaxel (HA-PTX) showed the distinguishing morphological features of apoptosis. Flow cytometric analysis showed that HA-PTX induces apoptosis as a significant mode of cell death. The activation level of tumor suppressor protein (p53) increased after PTX treatment in MCF-7, but no changes observed in HT-29 might be due to hereditary mutations. The lack of suppression in AKT and Rho A protein suggest the use of possible inhibitors in future studies which might could play a role in increasing the sensitivity of drug towards mutated cells line and reducing the possibilities for cancer cell survival, migration, and metastasis.
ISSN:2231-4040
0976-2094
DOI:10.4103/japtr.JAPTR_26_20