Antioxidant defenses of flame scallop Ctenoides scaber (Born, 1778) exposed to the water-soluble fraction of used vehicle crankcase oils

• Published in: Toxicology reports 2020-01, Vol.7, p.1597-1606
• Main Authors: Zapata-Vívenes, Edgar, Nusetti, Osmar, Marcano, Leida, Sánchez, Gabriela, Guderley, Helga
• Format: Article
• Language: English
• Subjects: Antioxidant enzymes; Catalase; Oxidative damage; Reduced glutathione; Regular; Water-soluble fraction
• ISSN: 2214-7500; 2214-7500
• DOI: 10.1016/j.toxrep.2020.11.009

[Display omitted] •This paper evaluated effects of a WSF-UVCO on the antioxidant responses of the scallop Lima scabra.•The antioxidant defenses in L. scabra seem be highly sensitive to low doses of to WSF-UVCO.•Digestive gland and gill show stronger antioxidant responses in L. scabra exposed to WSF-UVCO.•L. scabra could be good sensor for screening pollutant impacts along the Caribbean coastline. Used vehicle crankcase oils are a source of contamination in Caribbean marine environments and may alter the oxidative balance of organism that inhabiting coastal ecosystems. This paper aims to evaluate effects of a water-soluble fraction of used vehicle crankcase oils (WSF-UVCO) on the antioxidant responses of the flame scallop Ctenoides scaber. The organisms were exposed to ascending sublethal concentrations 0, 0.001, 0.01 and 0.1 % of WSF-UVCO in a static system of aquaria during one week. Subsequently activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione-S-transferase (GST) as well as concentrations of reduced glutathione (GSH) and thiobarbituric acid reactive substances (TBARS) were determined in the digestive gland, adductor muscle and gills. SOD, CAT, GST and TBARS increased in digestive gland of organisms exposed to WSF-UVCO at medium and highest concentrations, with a concomitant decrease in GPX and GR activities. In adductor muscle CAT decreased, but GR rose with exposure to 0.01 and 0.1 % WSF-UVCO; in gills, GST rose through all WSF-UVCO concentrations, and SOD, CAT and GR increased only at 0.1 %. The fluctuations in antioxidant enzymes and GST activities point out possible adjustments to control ROS production and detoxification of xenobiotics. These biochemical responses may guarantee the oxidative balance in flame scallop during short term exposure to low concentrations of WSF-UVCO. C. scaber appears suitable as an experimental organism for evaluating biological risks of sublethal exposure to hazardous xenobiotics in tropical marine environments.