Trdmt1 3'-untranslated region functions as a competing endogenous RNA in leukemia HL-60 cell differentiation

Severe blockage in myeloid differentiation is the hallmark of acute myeloid leukemia (AML). Trdmt1 plays an important role in hematopoiesis. However, little is known about the function of Trdmt1 in AML cell differentiation. In the present study, Trdmt1 was up-regulated and miR-181a was down-regulate...

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Bibliographic Details
Published in:Brazilian journal of medical and biological research 2021-01, Vol.54 (2), p.e9869-e9869
Main Authors: Xu, Sha, Xiong, Jun, Wu, Minjuan, Yang, Yu, Jiang, Junfeng, Ni, Haitao, Zhao, Yunpeng, Wang, Yue
Format: Article
Language:English
Subjects:
3' Untranslated Regions
Acute myeloid leukemia
BIOLOGY
C/EBPα
Cell Differentiation
Cell fusion
ceRNA
DNA (Cytosine-5-)-Methyltransferases - genetics
Fusion protein
Health aspects
HL-60 Cells
Humans
Leukemia
Leukemia, Myeloid, Acute - genetics
MEDICINE, RESEARCH & EXPERIMENTAL
MicroRNA
MicroRNAs - genetics
miRNA-181a
Myeloid leukemia
Myelopoiesis
Ribonucleic acid
RNA
Therapeutic targets
Trdmt1
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Summary:Severe blockage in myeloid differentiation is the hallmark of acute myeloid leukemia (AML). Trdmt1 plays an important role in hematopoiesis. However, little is known about the function of Trdmt1 in AML cell differentiation. In the present study, Trdmt1 was up-regulated and miR-181a was down-regulated significantly during human leukemia HL-60 cell differentiation after TAT-CT3 fusion protein treatment. Accordingly, miR-181a overexpression in HL-60 cells inhibited granulocytic maturation. In addition, our "rescue" assay demonstrated that Trdmt1 3'-untranslated region promoted myeloid differentiation of HL-60 cells by sequestering miR-181a and up-regulating C/EBPα (a critical factor for normal myelopoiesis) via its competing endogenous RNA (ceRNA) activity on miR-181a. These findings revealed an unrecognized role of Trdmt1 as a potential ceRNA for therapeutic targets in AML.
ISSN:0100-879X
1414-431X
1414-431X
1678-4510
DOI:10.1590/1414-431X20209869