Impacts of AlaAT3 transgenic poplar on rhizosphere soil chemical properties, enzyme activity, bacterial community, and metabolites under two nitrogen conditions

Poplar stands as one of the primary afforestation trees globally. We successfully generated transgenic poplar trees characterized by enhanced biomass under identical nutrient conditions, through the overexpression of the pivotal nitrogen assimilation gene, pxAlaAT3. An environmental risk assessment...

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Bibliographic Details
Published in:GM crops & food 2024-12, Vol.15 (1), p.170-184
Main Authors: Yang, Shengdong, Wang, Gang, Niu, Minghui, Zhang, Heng, Ma, Jing, Qu, Chunpu, Liu, Guanjun
Format: Article
Language:English
Subjects:
Biomass
Endopeptidases
Genetically modified
Nitrogen
Populus - genetics
populus × xiaohei
pxAlaAT3
Rhizosphere
rhizosphere soil
safety assessment
Soil
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Summary:Poplar stands as one of the primary afforestation trees globally. We successfully generated transgenic poplar trees characterized by enhanced biomass under identical nutrient conditions, through the overexpression of the pivotal nitrogen assimilation gene, pxAlaAT3. An environmental risk assessment was conducted for investigate the potential changes in rhizosphere soil associated with these overexpressing lines (OL). The results show that acid phosphatase activity was significantly altered under ammonium in OL compared to the wild-type control (WT), and a similar difference was observed for protease under nitrate. 16SrDNA sequencing indicated no significant divergence in rhizosphere soil microbial community diversity between WT and OL. Metabolomics analysis revealed that the OL caused minimal alterations in the metabolites of the rhizosphere soil, posing no potential harm to the environment. With these findings in mind, we anticipate that overexpressed plants will not adversely impact the surrounding soil environment.
ISSN:2164-5698
2164-5701
DOI:10.1080/21645698.2024.2339568