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Coelenterazine Indicators for the Specific Imaging of Human and Bovine Serum Albumins

Albumin assays in serum are important for the prognostic assessment of many life-threatening diseases, such as heart failure, liver disease, malnutrition, inflammatory bowel disease, infections, and kidney disease. In this study, synthetic coelenterazine (CTZ) indicators are developed to quantitativ...

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Published in:Sensors (Basel, Switzerland) Switzerland), 2023-06, Vol.23 (13), p.6020
Main Authors: Kim, Sung-Bae, Kamiya, Genta, Furuta, Tadaomi, Kitada, Nobuo, Maki, Shojiro A
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description Albumin assays in serum are important for the prognostic assessment of many life-threatening diseases, such as heart failure, liver disease, malnutrition, inflammatory bowel disease, infections, and kidney disease. In this study, synthetic coelenterazine (CTZ) indicators are developed to quantitatively illuminate human and bovine serum albumins (HSA and BSA) with high specificity. Their functional groups were chemically modified to specifically emit luminescence with HSA and BSA. The CTZ indicators were characterized by assaying the most abundant serum proteins and found that the CTZ indicators S6 and S6h were highly specific to HSA and BSA, respectively. Their colors were dramatically converted from blue, peaked at 480 nm, to yellowish green, peaked at 535 nm, according to the HSA-BSA mixing ratios, wherein the origins and mixing levels of the albumins can be easily determined by their colors and peak positions. The kinetic properties of HSA and BSA were investigated in detail, confirming that the serum albumins catalyze the CTZ indicators, which act as pseudo-luciferases. The catalytic reactions were efficiently inhibited by specific inhibitors, blocking the drug-binding sites I and II of HSA and BSA. Finally, the utility of the CTZ indicators was demonstrated through a quantitative imaging of the real fetal bovine serum (FBS). This study is the first example to show that the CTZ indicators specify HSA and BSA with different colors. This study contributes to the expansion of the toolbox of optical indicators, which efficiently assays serum proteins in physiological samples. Considering that these CTZ indicators immediately report quantitative optical signals with high specificity, they provide solutions to conventional technical hurdles on point-of-care assays of serum albumins.
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subjects Albumin
Assaying
Blood proteins
Cattle
coelenterazine
Disease
Forensic medicine
Functional groups
Gastrointestinal diseases
Health aspects
Humans
Imidazoles
Immunoglobulins
Indicators
Kidney diseases
Liver diseases
luminescence
Medical imaging
Medical research
Medicine, Experimental
Mixing ratio
Optical communication
Physiological aspects
Protein Binding
Proteins
Pyrazines
redshift
Scientific equipment and supplies industry
Serum albumin
Serum Albumin - chemistry
Serum Albumin, Bovine - chemistry
Serum Albumin, Human
Serum proteins
Spectrometry, Fluorescence
title Coelenterazine Indicators for the Specific Imaging of Human and Bovine Serum Albumins
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