Virus-Induced Gene Silencing in Chrysanthemum seticuspe Using the Tomato Aspermy Virus Vector

is one of the most economically important flowers globally due to its high ornamental value. In recent years, a large percentage of the genome has been determined, making this species useful as a model chrysanthemum plant. To fully utilize the genome's information, efficient and rapid gene func...

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Bibliographic Details
Published in:Plants (Basel) 2022-02, Vol.11 (3), p.430
Main Authors: Murai, Hirotomo, Mochizuki, Tomofumi
Format: Article
Language:English
Subjects:
Chrysanthemum
Cloning
Desaturase
Economic importance
Flowers
Functional analysis
Gene expression
Gene silencing
Genomes
Inoculation
Nucleotides
Phenotypes
Photobleaching
Plant viruses
Proteins
RNA polymerase
Seeds
tomato aspermy virus
Tomatoes
virus-induced gene silencing
Viruses
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Summary:is one of the most economically important flowers globally due to its high ornamental value. In recent years, a large percentage of the genome has been determined, making this species useful as a model chrysanthemum plant. To fully utilize the genome's information, efficient and rapid gene functional analysis methods are needed. In this study, we optimized the tomato aspermy virus (TAV) vector for virus-induced gene silencing (VIGS) in . Conventional plant virus inoculation methods, such as the mechanical inoculation of viral RNA transcripts and agroinoculation into leaves, did not achieve successful TAV infections in , but vacuum infiltration into sprouts was successful without symptoms. The TAV vector harboring 100 nucleotides of the ( ) gene caused photobleaching phenotypes and a reduction in expression in . To our knowledge, this is the first report of VIGS in chrysanthemums.
ISSN:2223-7747
2223-7747
DOI:10.3390/plants11030430