The ameliorative role of specific probiotic combinations on bone loss in the ovariectomized rat model

Background Osteoporosis, a skeletal disease described by impaired bone strength, cause an increased risk of fractures. We aimed in this study to clarify which particular wise combination of probiotics has the most beneficial effect in the rat model of osteoporosis. Methods Sixty-three mature female...

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Published in:BMC complementary and alternative medicine 2022-09, Vol.22 (1), p.1-241, Article 241
Main Authors: Gholami, Ahmad, Dabbaghmanesh, Mohammad Hossein, Ghasemi, Younes, Koohpeyma, Farhad, Talezadeh, Pedram, Montazeri-Najafabady, Nima
Format: Article
Language:English
Subjects:
Alfacalcidol
Alkaline phosphatase
Bacteria
Biochemical markers
Bone
Bone density
Bone loss
Bone mineral content
Bone mineral density
Bone strength
Bones
Calcifediol
Calciferol
Calcium
Calcium (blood)
Density
Dual energy X-ray absorptiometry
Femur
Fermented food
Fractures
Immune system
Laboratory animals
Lactic acid
Lactic acid bacteria
Lactobacilli
Lactobacillus
Medical research
Osteoporosis
Ovariectomy
Phosphatases
Phosphorus
Probiotic
Probiotics
Rodents
Spine
Statistical analysis
Surgical anastomosis
Tibia
Vitamin D
Yogurt
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Summary:Background Osteoporosis, a skeletal disease described by impaired bone strength, cause an increased risk of fractures. We aimed in this study to clarify which particular wise combination of probiotics has the most beneficial effect in the rat model of osteoporosis. Methods Sixty-three mature female Sprague Dawley rats (12-14 weeks old, weight 200 [+ or -] 20 g) were ovariectomized and then divided into nine random groups, each group consisting of 7 rats. Lactic acid bacteria were isolated from traditional fermented yogurt on the northern coast of the Persian Gulf. Seven combinations of probiotics, each containing three probiotic strains, were designed and administered (1 x 10 .sup.9 CFU / ml/strain daily along with their water) to treat ovariectomized rats. The period from ovariectomy to eutanásia was 3 months. For evaluating femur, spine, and tibia, bone mineral density (BMD), and bone mineral content (BMC), Dual-energy X-ray absorptiometry (DEXA) scans were performed. Also, effect of probiotic combinations was assessed on biochemical markers including vitamin D, calcium, phosphorus, and alkaline phosphatase in serum. Results Combination NO 4, containing L. acidophilus, B. longum, and L. reuteri, is the most influential group on global, spine, and femur BMD. Combination NO 3, containing L. acidophilus, L. casei, and L. reuteri, also significantly affects the BMD of the tibia among the treatment group. We found that the combination NO 4 had the most significant ameliorative effect on global BMC. Also, combination NO 1 (comprising L. acidophilus, L. casei, and B. longum), NO 6 (containing L. casei, B. longum, and Bacillus coagulans), NO 7 (containing L. casei, L. reuteri, and B. longum), and NO 4 had the most considerable raising effect on spine BMC. In addition, the serum calcium and Vitamin D concentration in the groups NO 4, 6, and 7 were significantly higher than in OVX groups, whereas the alkaline phosphatase concentration was considerably reduced in these groups. Conclusion Among nine effective probiotics, a combination containing L. acidophilus, B. longum, and L. reuteri is the most influential group in ovariectomized osteoporotic rat. Keywords: Probiotic, Osteoporosis, Lactobacillus, Bone
ISSN:2662-7671
2662-7671
1472-6882
DOI:10.1186/s12906-022-03713-y