Coal-straw co-digestion-induced biogenic methane production: perspectives on microbial communities and associated metabolic pathways

• Published in: Scientific reports 2024-11, Vol.14 (1), p.26554-13, Article 26554
• Main Authors: Khan, Sohail, Deng, Ze, Wang, Bobo, Yu, Zhisheng
• Format: Article
• Language: English
• Subjects: 631/326; 631/61; 704/172; Anaerobic co-digestion; Anaerobic digestion; Anaerobic microorganisms; Aromatic compounds; Bacteria - metabolism; Bioavailability; Biomethane; Carbon dioxide; Coal; Crop yield; Humanities and Social Sciences; Metabolic Networks and Pathways; Metabolic pathways; Metabolism; Metabolites; Methane; Methane - metabolism; Methanobacteriaceae - metabolism; Microbial activity; Microbial communities; Microbiomes; Microbiota; multidisciplinary; Phthalic acid; Science; Science (multidisciplinary); Straw; Triticum - metabolism; Wheat straw
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-75655-z

This study assessed the impacts of wheat straw as a cosubstrate on coal biocoverion into methane and the associated mechanism within methane metabolic pathways. Co-digestion of coal with varying wheat straw concentrations resulted in a remarkable (1246.05%) increase in methane yield compared to that of the control (CK). Moreover, microbial analysis revealed a uniform distribution of Methanosarcinaceae (51.14%) and Methanobacteriaceae (39.90%) in the co-digestion of coal and wheat straw (CWS1) at a ratio of 3:1 (w/w) compared to other treatments such as coal and wheat straw (CWS2) at a ratio of 3:0.5. In addition, Hungatieclostridiaceae and Rhodobacteriaceae were abundant in both co-digesters, whereas the bacterial communities in the CK group were significantly different and more abundant than those in the Peptostreptococcaceae and Enterobacteriaceae groups. The key enzymes related to methanogenic metabolic pathways, including EC: 1.2.99.5 and EC: 2.1.1.86 (facilitating the conversion of CO 2 into methane), and EC:1.12.98.1 exhibited significant abundance within CWS1. Aromatic compounds such as 4-(2-chloroanilino)-4-oxobutanoic acid and phthalic acid were substantially more abundant in CWS1 and CWS2 than in CK, indicating the increased bioavailability of coal to microbial activities. This novel approach demonstrates that wheat straw co-digestion with coal during anaerobic digestion modulates microbial communities and their metabolic pathways to enhance methane production from complex substrates such as coal.