Epigallocatechin Gallate-Modified Gelatins with Different Compositions Alter the Quality of Regenerated Bones

Bone quality is a significant indicator of the result of bone treatments. However, information regarding the quality of regenerated bones is limited. The study investigates the effect of different compositions of vacuum heated epigallocatechin gallate-modified gelatins sponge (vhEGCG-GS) on the qual...

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Bibliographic Details
Published in:International journal of molecular sciences 2018-10, Vol.19 (10), p.3232
Main Authors: Hara, Eiki, Honda, Yoshitomo, Suzuki, Osamu, Tanaka, Tomonari, Matsumoto, Naoyuki
Format: Article
Language:English
Subjects:
Animals
Bone Density
bone formation
bone quality
Bone Regeneration
Bone Substitutes - chemistry
Bone Substitutes - therapeutic use
Catechin - analogs & derivatives
Catechin - chemistry
collagen
EGCG
FTIR imaging
gelatin
Gelatin - chemistry
Guided Tissue Regeneration - methods
Male
picrosirius red staining
polarized microscope
Rats
Rats, Sprague-Dawley
regenerated bone
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Summary:Bone quality is a significant indicator of the result of bone treatments. However, information regarding the quality of regenerated bones is limited. The study investigates the effect of different compositions of vacuum heated epigallocatechin gallate-modified gelatins sponge (vhEGCG-GS) on the quality of regenerated bones in critical size defects (9 mm) of rat calvariae. Five different compositions of vhEGCG-GSs containing the same amount of EGCG and different amounts of gelatin were tested. Following four weeks after implantation, the harvested regenerated bones were evaluated by using micro-computed tomography analysis, histological evaluation (hematoxylin-eosin and Villaneueva Goldner staining), picrosirius red-staining with polarized microscopic observation for collagen maturation, and Fourier transform infrared spectroscopy microscopy and imaging analysis for mineral-matrix ratio. The results indicated that increasing content of gelatin in the vhEGCG-GSs promoted bone and osteoid formation but yielded porous bones. Furthermore, tissue mineral density decreased and the maximum mineral-matrix ratio increased. In contrast, vhEGCG-GSs containing smaller amount of gelatin formed mature collagen matrix in the regenerated bones. These results suggest that the alteration of composition of vhEGCG-GSs affected the bone forming capability and quality of regenerated bone and provides valuable insight for the fabrication of new bone substitute materials.
ISSN:1422-0067
1422-0067
DOI:10.3390/ijms19103232