DNA Multi-Marker Genotyping and CIAS Morphometric Phenotyping of Fasciola gigantica-Sized Flukes from Ecuador, with an Analysis of the Radix Absence in the New World and the Evolutionary Lymnaeid Snail Vector Filter

Fascioliasis is a disease caused by Fasciola hepatica worldwide transmitted by lymnaeid snails mainly of the Galba/Fossaria group and F. gigantica restricted to parts of Africa and Asia and transmitted by Radix lymnaeids. Concern has recently risen regarding the high pathogenicity and human infectio...

Full description

Saved in:
Bibliographic Details
Published in:Animals (Basel) 2021-08, Vol.11 (9), p.2495
Main Authors: Bargues, Maria Dolores, Valero, Maria Adela, Trueba, Gabriel A., Fornasini, Marco, Villavicencio, Angel F., Guamán, Rocío, De Elías-Escribano, Alejandra, Pérez-Crespo, Ignacio, Artigas, Patricio, Mas-Coma, Santiago
Format: Article
Language:English
Subjects:
Altitude
Biological evolution
Cattle
Climate change
Deoxyribonucleic acid
Disease
DNA
Evolutionary genetics
F. gigantica
Fasciola hepatica
Fragments
genotypic DNA characterization
Genotyping
Haplotypes
Hybridization
Hybrids
Infections
ITS-2
Liver
Livestock
Mitochondrial DNA
morphometry by CIAS
Nucleotide sequence
Parasites
Pathogenicity
Pathogens
phenotypic characterization
Phenotyping
Populations
Public health
Ribosomal DNA
Rural areas
Sheep
Snails
Tropical diseases
Uterus
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Fascioliasis is a disease caused by Fasciola hepatica worldwide transmitted by lymnaeid snails mainly of the Galba/Fossaria group and F. gigantica restricted to parts of Africa and Asia and transmitted by Radix lymnaeids. Concern has recently risen regarding the high pathogenicity and human infection capacity of F. gigantica. Abnormally big-sized fasciolids were found infecting sheep in Ecuador, the only South American country where F. gigantica has been reported. Their phenotypic comparison with F. hepatica infecting sheep from Peru, Bolivia and Spain, and F. gigantica from Egypt and Vietnam demonstrated the Ecuadorian fasciolids to have size-linked parameters of F. gigantica. Genotyping of these big-sized fasciolids by rDNA ITS-2 and ITS-1 and mtDNA cox1 and nad1 and their comparison with other countries proved the big-sized fasciolids to belong to F. hepatica. Neither heterozygotic ITS position differentiated the two species, and no introgressed fragments and heteroplasmic positions in mtDNA were found. The haplotype diversity indicates introductions mainly from other South American countries, Europe and North America. Big-sized fasciolids from Ecuador and USA are considered to be consequences of F.gigantica introductions by past livestock importations. The vector specificity filter due to Radix absence should act as driving force in the evolution in such lineages.
ISSN:2076-2615
2076-2615
DOI:10.3390/ani11092495