Chemically different non-thermal plasmas target distinct cell death pathways

A rigorous biochemical analysis of interactions between non-thermal plasmas (NTPs) and living cells has become an important research topic, due to recent developments in biomedical applications of non-thermal plasmas. Here, we decouple distinct cell death pathways targeted by chemically different NT...

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Bibliographic Details
Published in:Scientific reports 2017-04, Vol.7 (1), p.600-16, Article 600
Main Authors: Lunov, Oleg, Zablotskii, Vitalii, Churpita, Olexander, Lunova, Mariia, Jirsa, Milan, Dejneka, Alexandr, Kubinová, Šárka
Format: Article
Language:English
Subjects:
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Apoptosis
Biochemical analysis
Cell death
Gangrene
Helium
Humanities and Social Sciences
Mitochondria
Mortality
multidisciplinary
Necroptosis
Necrosis
Permeability
Phagocytosis
Reactive oxygen species
Science
Science (multidisciplinary)
TOR protein
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Summary:A rigorous biochemical analysis of interactions between non-thermal plasmas (NTPs) and living cells has become an important research topic, due to recent developments in biomedical applications of non-thermal plasmas. Here, we decouple distinct cell death pathways targeted by chemically different NTPs. We show that helium NTP cells treatment, results in necrosome formation and necroptosis execution, whereas air NTP leads to mTOR activation and autophagy inhibition, that induces mTOR-related necrosis. On the contrary, ozone (abundant component of air NTP) treatment alone, exhibited the highest levels of reactive oxygen species production leading to CypD-related necrosis via the mitochondrial permeability transition. Our findings offer a novel insight into plasma-induced cellular responses, and reveal distinct cell death pathways triggered by NTPs.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-00689-5