Preliminary Characterization, Antioxidant and Hepatoprotective Activities of Polysaccharides from Taishan Pinus massoniana Pollen

• Published in: Molecules (Basel, Switzerland) Switzerland), 2018-01, Vol.23 (2), p.281
• Main Authors: Zhou, Changming, Yin, Shaojie, Yu, Zhongfang, Feng, Yuxiang, Wei, Kai, Ma, Weiming, Ge, Lijiang, Yan, Zhengui, Zhu, Ruiliang
• Format: Article
• Language: English
• Subjects: Alanine; Alanine transaminase; Alkaline phosphatase; Animal tissues; Animals; antioxidant activity; Antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Arabinose; Aspartate aminotransferase; Carbon tetrachloride; Carbon Tetrachloride Poisoning - metabolism; Carbon Tetrachloride Poisoning - pathology; Carbon Tetrachloride Poisoning - prevention & control; characterization; Composition effects; Dose-Response Relationship, Drug; Fourier transforms; Free radicals; Glutathione peroxidase; hepatoprotective effect; Hepatotoxicity; High-performance liquid chromatography; Infrared absorption; Infrared analysis; Infrared spectra; Infrared spectroscopy; Lipid peroxidation; Liquid chromatography; Liver; Liver - metabolism; Liver - pathology; Male; Malondialdehyde; Monosaccharides; Oxidative stress; Peroxidase; Peroxidation; Pine trees; Pinus - chemistry; Pinus massoniana; Pollen; Pollen - chemistry; Polysaccharides; Polysaccharides - chemistry; Polysaccharides - pharmacology; Pretreatment; Rats; Rats, Wistar; Rodents; Saccharides; Spectrum analysis; Superoxide dismutase; Taishan Pinus massoniana pollen
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules23020281

The objectives of the present study were to characterize the chemical composition, antioxidant activity and hepatoprotective effect of the polysaccharides from Taishan pollen (TPPPS). HPLC analysis showed that TPPPS was an acidic heteropolysaccharide with glucose and arabinose as the main component monosaccharides (79.6%, molar percentage). Fourier transform-infrared spectroscopy (FT-IR) analysis indicated that the spectra of TPPPS displayed infrared absorption peaks characteristic of polysaccharides. In in vitro assays TPPPS exhibited different degrees of dose-dependent antioxidant activities , and this was further verified by suppression of CCl₄-induced oxidative stress in the liver with three tested doses of TPPPS (100, 200, and 400 mg/kg bw) in rats. Pretreatment with TPPPS significantly decreased the levels of alanine aminotransferase (AST), aspartate aminotransferase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH) and malondialdehyde (MDA) against CCl₄ injuries, and elevated the activities of superoxide dismutase (SOD) as well as glutathione peroxidase (GSH-Px). Histopathological observation further confirmed that TPPPS could protect the liver tissues from CCl₄-induced histological alternation. These results suggest that TPPPS has strong antioxidant activities and significant protective effect against acute hepatotoxicity induced by CCl₄. The hepatoprotective effect may partly be related to its free radical scavenging effect, increasing antioxidant activity and inhibiting lipid peroxidation.