Somatostatin-Mediated Regulation of Retinoic Acid-Induced Differentiation of SH-SY5Y Cells: Neurotransmitters Phenotype Characterization

During brain development, neurite formation plays a critical role in neuronal communication and cognitive function. In the present study, we compared developmental changes in the expression of crucial markers that govern the functional activity of neurons, including somatostatin (SST), choline acety...

Full description

Saved in:
Bibliographic Details
Published in:Biomedicines 2022-02, Vol.10 (2), p.337
Main Authors: Singh, Sneha, Somvanshi, Rishi K, Kumar, Ujendra
Format: Article
Language:English
Subjects:
Acetyltransferase
Acids
Axonogenesis
Cell differentiation
Cell growth
Choline O-acetyltransferase
Cognitive ability
Genotype & phenotype
Glutamate decarboxylase
Glutamic acid
Hydroxylase
Kinases
Morphology
Nervous system
Neural networks
neurite outgrowth
Neuroblastoma
Neurodegeneration
Neurogenesis
Neurons
neurotransmitter
Neurotransmitters
Nitric-oxide synthase
Phenotypes
Proteins
Retinoic acid
SH-SY5Y cells
Somatostatin
Synaptophysin
Transcription factors
Tyrosine 3-monooxygenase
γ-Aminobutyric acid
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:During brain development, neurite formation plays a critical role in neuronal communication and cognitive function. In the present study, we compared developmental changes in the expression of crucial markers that govern the functional activity of neurons, including somatostatin (SST), choline acetyltransferase (ChAT), tyrosine hydroxylase (TH), brain nitric oxide synthase (bNOS), gamma-aminobutyric acid (GABA), glutamic acid decarboxylase (GAD-65) and synaptic vesicle protein synaptophysin (SYP) in non-differentiated and retinoic acid (RA)-induced differentiated SH-SY5Y cells. We further determined the role of SST in regulating subcellular distribution and expression of neurotransmitters. Our results indicate that SST potentiates RA-induced differentiation of SH-SY5Y cells and involves regulating the subcellular distribution and expression of neurotransmitter markers and synaptophysin translocation to neurites in a time-dependent manner, anticipating the therapeutic implication of SST in neurodegeneration.
ISSN:2227-9059
2227-9059
DOI:10.3390/biomedicines10020337