Overexpression of the Oral Mucosa-Specific microRNA-31 Promotes Skin Wound Closure

Wounds within the oral mucosa are known to heal more rapidly than skin wounds. Recent studies suggest that differences in the microRNAome profiles may underlie the exceptional healing that occurs in oral mucosa. Here, we test whether skin wound-healing can be accelerating by increasing the levels of...

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Published in:International journal of molecular sciences 2019-07, Vol.20 (15), p.3679
Main Authors: Chen, Lin, Simões, Alyne, Chen, Zujian, Zhao, Yan, Wu, Xinming, Dai, Yang, DiPietro, Luisa A, Zhou, Xiaofeng
Format: Article
Language:English
Subjects:
Animals
Cell adhesion & migration
Cell lines
Chi-square test
Cluster analysis
Computational Biology - methods
Datasets
Epithelial cells
Epithelial Cells - metabolism
Female
Gene Expression
Gene Expression Profiling
Gene Expression Regulation
Humans
Mice
microRNA
MicroRNAs
MicroRNAs - genetics
miR-31
miRNA
Mouth Mucosa - metabolism
Mucosa
oral mucosal wound
Skin
Skin - metabolism
skin wound
Statistical analysis
Statistical tests
Variance analysis
Wound healing
Wound Healing - genetics
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Summary:Wounds within the oral mucosa are known to heal more rapidly than skin wounds. Recent studies suggest that differences in the microRNAome profiles may underlie the exceptional healing that occurs in oral mucosa. Here, we test whether skin wound-healing can be accelerating by increasing the levels of oral mucosa-specific microRNAs. A panel of 57 differentially expressed high expresser microRNAs were identified based on our previously published miR-seq dataset of paired skin and oral mucosal wound-healing [Sci. Rep. (2019) 9:7160]. These microRNAs were further grouped into 5 clusters based on their expression patterns, and their differential expression was confirmed by TaqMan-based quantification of LCM-captured epithelial cells from the wound edges. Of these 5 clusters, Cluster IV (consisting of 8 microRNAs, including miR-31) is most intriguing due to its tissue-specific expression pattern and temporal changes during wound-healing. The in vitro functional assays show that ectopic transfection of miR-31 consistently enhanced keratinocyte proliferation and migration. In vivo, miR-31 mimic treatment led to a statistically significant acceleration of wound closure. Our results demonstrate that wound-healing can be enhanced in skin through the overexpression of microRNAs that are highly expressed in the privileged healing response of the oral mucosa.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms20153679