A Polyamidoamine-Based Electrochemical Aptasensor for Sensitive Detection of Ochratoxin A

Sensitive detection of ochratoxin A (OTA) is significant and essential because OTA may pose risks to human and animal health. Here, we developed an electrochemical aptasensor for OTA analysis using polyamidoamine (PAMAM) dendrimers as a signal amplifier. As a carrier, PAMAM has numerous primary amin...

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Bibliographic Details
Published in:Biosensors (Basel) 2023-10, Vol.13 (11), p.955
Main Authors: Chen, Xiujin, Gao, Dong, Chen, Jiaqi, Wang, Xueqing, Peng, Chifang, Gao, Hongli, Wang, Yao, Li, Zhaozhou, Niu, Huawei
Format: Article
Language:English
Subjects:
Agricultural products
Amino groups
Animal health
Aptamers
AuNPs
Biosensors
Carbon
Chemical properties
Chromatography
Contamination
Dendrimers
Deoxyribonucleic acid
Design and construction
DNA
Electrochemical apparatus
electrochemical aptasensor
Electrochemistry
Electrodes
Electrons
Farm produce
Gold
Graphene
graphene oxide
Health aspects
Horseradish peroxidase
Measurement
Mycotoxins
Nanocomposites
Nanomaterials
Nanoparticles
Nucleic acid aptamers
Ochratoxin A
PAMAM
Peroxidase
Signal monitoring
Storage stability
Transmission electron microscopy
Wines
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Summary:Sensitive detection of ochratoxin A (OTA) is significant and essential because OTA may pose risks to human and animal health. Here, we developed an electrochemical aptasensor for OTA analysis using polyamidoamine (PAMAM) dendrimers as a signal amplifier. As a carrier, PAMAM has numerous primary amino groups that can be coupled with thiolated complementary strand DNA (cDNA), allowing it to recognize aptamers bound to the surface of horseradish peroxidase (HRP)-modified gold nanoparticles (AuNPs), thereby improving the sensitivity of the aptasensor. When monitoring the positive samples, OTA was captured by the aptamer fixed on the HRP-conjugated AuNP surface by specific recognition, after which the formed OTA-aptamer conjugates were detached from the electrode surface, ultimately decreasing the electrochemical signal monitored by differential pulse voltammetry. The novel aptasensor achieved a broad linear detection range from 5 to 105 ng L−1 with a low detection limit of 0.31 ng L−1. The proposed aptasensor was successfully applied for OTA analysis in red wine, with recovery rates ranging from 94.15 to 106%. Furthermore, the aptasensor also exhibited good specificity and storage stability. Therefore, the devised aptasensor represents a sensitive, practical and reliable tool for monitoring OTA in agricultural products, which can also be adapted to other mycotoxins.
ISSN:2079-6374
2079-6374
DOI:10.3390/bios13110955