Curcumin Ameliorate Diabetes type 1 Complications through Decreasing Pro-inflammatory Cytokines in C57BL/6 Mice

• Published in: Iranian journal of allergy, asthma, and immunology asthma, and immunology, 2020-05, Vol.19 (S1), p.55
• Main Authors: Yaser Jafari Khataylou, Ahmadiafshar, Somayeh, Rezaei, Reza, Parsamanesh, Saeid, Hosseini, Golbahar
• Format: Article
• Language: English
• Subjects: Anti-inflammatory agents; Autoimmune diseases; Beta cells; C-peptide; Citric acid; Curcumin; Diabetes; Diabetes mellitus (insulin dependent); Enzyme-linked immunosorbent assay; Inbreeding; Inflammation; Insulin; Interleukin 10; Interleukin 17; Interleukin 6; Islets of Langerhans; Mice; Peptides; Radioimmunoassay; Serum levels; Splenocytes; Tumor necrosis factor-TNF; Tumor necrosis factor-α; Type 1 diabetes mellitus; γ-Interferon
• ISSN: 1735-1502; 1735-5249
• DOI: 10.18502/ijaai.v19i(s1.r1).2854

Type 1 diabetes is a chronic autoimmune disease of beta cells in the islets of Langerhans, which are responsible for making insulin. Even with insulin therapy, inflammatory complications will develop in the long term. The present study examines changes in serum levels of interleukin (IL)-6, IL-17, IL-10, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, C-peptide, Insulin as well as fasting blood sugar (FBS) in control, diabetic and diabetic treated with curcumin groups. Thirty inbred C57BL /6 mice were randomly divided into three groups of 10 mice: group A consisted of healthy mice receiving citrate buffer, group B included a group of diabetic mice, and group C was a group of diabetic mice treated with curcumin. The cytokine levels were measured in the supernatant of stimulated splenocytes using enzyme -linked immunosorbent assay (ELISA). Radioimmunoassay was used to measure insulin and c-peptide levels. The FBS was measured by an automatic glucometer device. The levels of IL-6, IL-17, and IFN-γ, as well as FBS, was significantly decreased in the treated group with curcumin compared to the diabetic group mice (p