Rapid Identification of the Multiple Absorbed Bioactive Components and Metabolites in Rat Serum after Oral Administration of Wu-Jia Sheng-Hua Capsule by UPLC-ESI-MS

To identify the compounds absorbed in rat serum after the oral administration of Wu-Jia Sheng-Hua (WJSH) capsule, a traditional Chinese medicine (TCM) compound prescription, an ultraperformance liquid chromatography coupled with electrospray ionization mass spectrometry (UPLC/ESI-MS) method, was est...

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Bibliographic Details
Published in:Journal of analytical methods in chemistry 2013-01, Vol.2013 (2013), p.1-6
Main Authors: Geng, Fang, Wang, Fashan, Zou, Taobo, Zhu, Kuiyuan, Ma, Wei, Tan, Guanghui, Zhang, Ning
Format: Article
Language:English
Subjects:
Acetonitrile
Buffers
Chinese medicine
Chromatography
Ionization
Mathematical analysis
Metabolites
Postpartum period
Rodents
Serums
Traditional chinese medicine
Womens health
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Summary:To identify the compounds absorbed in rat serum after the oral administration of Wu-Jia Sheng-Hua (WJSH) capsule, a traditional Chinese medicine (TCM) compound prescription, an ultraperformance liquid chromatography coupled with electrospray ionization mass spectrometry (UPLC/ESI-MS) method, was established. The chromatographic separation of the absorbed compounds and metabolites was achieved with an ACQUITY UPLC BEH C18 column (2.1 mm × 50 mm, 1.7 μm) under a gradient elution. The mobile phase was composed of acetonitrile and water buffered with ammonium acetate (10 mM) and formic acid (0.1%, V/V). Twelve absorbed compounds and four metabolites were found. Seven of the absorbed compounds were identified by ESI-MS. The identification of absorbed compounds might be helpful for the better understanding of the mechanisms underlying the pharmacological effects of WJSH capsule.
ISSN:2090-8865
2090-8873
DOI:10.1155/2013/318961