Non-Productive Infection of Glial Cells with SARS-CoV-2 in Hamster Organotypic Cerebellar Slice Cultures

The numerous neurological syndromes associated with COVID-19 implicate an effect of viral pathogenesis on neuronal function, yet reports of direct SARS-CoV-2 infection in the brain are conflicting. We used a well-established organotypic brain slice culture to determine the permissivity of hamster br...

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Bibliographic Details
Published in:Viruses 2022-06, Vol.14 (6), p.1218
Main Authors: Lamoureux, Lise, Sajesh, Babu, Slota, Jessy A., Medina, Sarah J., Mayor, Matthew, Frost, Kathy L., Warner, Bryce, Manguiat, Kathy, Wood, Heidi, Kobasa, Darwyn, Booth, Stephanie A.
Format: Article
Language:English
Subjects:
Antigens
astrocytes
Brain
Brain slice preparation
Cell culture
Cerebellum
Coronaviruses
COVID-19
Disease
Glial cells
Glucose
Infections
Inoculation
microglia
organotypic culture
Pathology
Phenotypes
SARS-CoV-2
Severe acute respiratory syndrome coronavirus 2
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Summary:The numerous neurological syndromes associated with COVID-19 implicate an effect of viral pathogenesis on neuronal function, yet reports of direct SARS-CoV-2 infection in the brain are conflicting. We used a well-established organotypic brain slice culture to determine the permissivity of hamster brain tissues to SARS-CoV-2 infection. We found levels of live virus waned after inoculation and observed no evidence of cell-to-cell spread, indicating that SARS-CoV-2 infection was non-productive. Nonetheless, we identified a small number of infected cells with glial phenotypes; however, no evidence of viral infection or replication was observed in neurons. Our data corroborate several clinical studies that have assessed patients with COVID-19 and their association with neurological involvement.
ISSN:1999-4915
1999-4915
DOI:10.3390/v14061218