A gustatory receptor tuned to the steroid plant hormone brassinolide in Plutella xylostella (Lepidoptera: Plutellidae)

• Published in: eLife 2020-12, Vol.9
• Main Authors: Yang, Ke, Gong, Xin-Lin, Li, Guo-Cheng, Huang, Ling-Qiao, Ning, Chao, Wang, Chen-Zhu
• Format: Article
• Language: English
• Subjects: Animals; brassinolide; Brassinosteroids - metabolism; Ecology; feeding; Female; functional analysis; gustatory receptor; Larva - physiology; Moths - physiology; Oviposition - physiology; Phytosterols - analysis; Phytosterols - metabolism; Plant Growth Regulators - analysis; Plant Growth Regulators - metabolism; Plant Leaves; Plutella xylostella; RNAi; Steroids, Heterocyclic - metabolism
• ISSN: 2050-084X; 2050-084X
• DOI: 10.7554/eLife.64114

Feeding and oviposition deterrents help phytophagous insects to identify host plants. The taste organs of phytophagous insects contain bitter gustatory receptors (GRs). To explore their function, the GRs in were analyzed. Through RNA sequencing and qPCR, we detected abundant transcripts in the larval head and adult antennae. Functional analyses using the oocyte expression system and 24 diverse phytochemicals showed that PxylGr34 is tuned to the canonical plant hormones brassinolide (BL) and 24-epibrassinolide (EBL). Electrophysiological analyses revealed that the medial sensilla styloconica of 4 instar larvae are responsive to BL and EBL. Dual-choice bioassays demonstrated that BL inhibits larval feeding and female oviposition. Knock-down of PxylGr34 by RNAi attenuates the taste responses to BL, and abolishes BL-induced feeding inhibition. These results increase our understanding of how herbivorous insects detect compounds that deter feeding and oviposition, and may be useful for designing plant hormone-based pest management strategies.