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Occurrence of Enterococci in the Process of Artisanal Cheesemaking and Their Antimicrobial Resistance
Enterococci are a group of microorganisms that have a controversial position from some scientific points of view. The species of the greatest clinical importance are and , which are common agents of nosocomial infections. However, enterococci also have important applications in the dairy industry, a...
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| Published in: | Life (Basel, Switzerland) Switzerland), 2024-07, Vol.14 (7), p.890 |
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| container_issue | 7 |
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| container_title | Life (Basel, Switzerland) |
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| creator | Hanzelová, Zuzana Dudriková, Eva Lovayová, Viera Výrostková, Jana Regecová, Ivana Zigo, František Bartáková, Klára |
| description | Enterococci are a group of microorganisms that have a controversial position from some scientific points of view. The species of the greatest clinical importance are
and
, which are common agents of nosocomial infections. However, enterococci also have important applications in the dairy industry, as they are used as non-starter lactic acid bacteria (NSLAB) in a variety of cheeses, especially artisanal cheeses. The aim of this study was to determine the presence of representatives from the
genus using PCR and MALDI-TOF MS methods on samples of raw milk, processing environment swabs, and cheese from four different artisanal dairy plants in Slovakia. Among the 136 isolates of enterococci, 9 species of genus
(
,
,
,
,
,
,
,
, and
) were identified and were tested for their antimicrobial resistance (AMR) to 8 antibiotics (amoxicillin, penicillin, ampicillin, erythromycin, levofloxacin, vancomycin, rifampicin, and tetracycline); most of them were resistant to rifampicin (35.3%), ampicillin (22.8%), and tetracycline (19.9%). A PCR analysis of
(4.41%) and
(14.71%) revealed that antimicrobial resistance genes were present in not only phenotypic resistant isolates of enterococci but also susceptible isolates. The investigation of antimicrobial resistance in enterococci during the cheesemaking process can be a source of valuable information for public health in the concept of "One Health". |
| doi_str_mv | 10.3390/life14070890 |
| format | article |
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and
, which are common agents of nosocomial infections. However, enterococci also have important applications in the dairy industry, as they are used as non-starter lactic acid bacteria (NSLAB) in a variety of cheeses, especially artisanal cheeses. The aim of this study was to determine the presence of representatives from the
genus using PCR and MALDI-TOF MS methods on samples of raw milk, processing environment swabs, and cheese from four different artisanal dairy plants in Slovakia. Among the 136 isolates of enterococci, 9 species of genus
(
,
,
,
,
,
,
,
, and
) were identified and were tested for their antimicrobial resistance (AMR) to 8 antibiotics (amoxicillin, penicillin, ampicillin, erythromycin, levofloxacin, vancomycin, rifampicin, and tetracycline); most of them were resistant to rifampicin (35.3%), ampicillin (22.8%), and tetracycline (19.9%). A PCR analysis of
(4.41%) and
(14.71%) revealed that antimicrobial resistance genes were present in not only phenotypic resistant isolates of enterococci but also susceptible isolates. The investigation of antimicrobial resistance in enterococci during the cheesemaking process can be a source of valuable information for public health in the concept of "One Health".</description><identifier>ISSN: 2075-1729</identifier><identifier>EISSN: 2075-1729</identifier><identifier>DOI: 10.3390/life14070890</identifier><identifier>PMID: 39063643</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Amoxicillin ; Ampicillin ; Antibiotics ; Antimicrobial agents ; Antimicrobial resistance ; Bacteria ; Cheese ; Dairy industry ; Dairy products ; Drug resistance ; enterococcal isolates ; Erythromycin ; Genes ; Information processing ; Lactic acid ; Lactic acid bacteria ; Levofloxacin ; MALDI-TOF MS ; Microorganisms ; Milk ; Nosocomial infection ; Pasteurization ; PCR ; Penicillin ; Plasmids ; processing environment ; Public health ; Rifampin ; silent genes ; Vancomycin ; Veterinary medicine</subject><ispartof>Life (Basel, Switzerland), 2024-07, Vol.14 (7), p.890</ispartof><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c310t-e447af10eb8a55d347d94aa61bd14734475a4ebe9369491e1e2a50aaed3a26a73</cites><orcidid>0000-0003-0190-4346 ; 0000-0002-3117-8915 ; 0000-0002-2791-166X ; 0000-0002-7393-1960</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3084930758/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3084930758?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,25753,27924,27925,37012,37013,44590,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39063643$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hanzelová, Zuzana</creatorcontrib><creatorcontrib>Dudriková, Eva</creatorcontrib><creatorcontrib>Lovayová, Viera</creatorcontrib><creatorcontrib>Výrostková, Jana</creatorcontrib><creatorcontrib>Regecová, Ivana</creatorcontrib><creatorcontrib>Zigo, František</creatorcontrib><creatorcontrib>Bartáková, Klára</creatorcontrib><title>Occurrence of Enterococci in the Process of Artisanal Cheesemaking and Their Antimicrobial Resistance</title><title>Life (Basel, Switzerland)</title><addtitle>Life (Basel)</addtitle><description>Enterococci are a group of microorganisms that have a controversial position from some scientific points of view. The species of the greatest clinical importance are
and
, which are common agents of nosocomial infections. However, enterococci also have important applications in the dairy industry, as they are used as non-starter lactic acid bacteria (NSLAB) in a variety of cheeses, especially artisanal cheeses. The aim of this study was to determine the presence of representatives from the
genus using PCR and MALDI-TOF MS methods on samples of raw milk, processing environment swabs, and cheese from four different artisanal dairy plants in Slovakia. Among the 136 isolates of enterococci, 9 species of genus
(
,
,
,
,
,
,
,
, and
) were identified and were tested for their antimicrobial resistance (AMR) to 8 antibiotics (amoxicillin, penicillin, ampicillin, erythromycin, levofloxacin, vancomycin, rifampicin, and tetracycline); most of them were resistant to rifampicin (35.3%), ampicillin (22.8%), and tetracycline (19.9%). A PCR analysis of
(4.41%) and
(14.71%) revealed that antimicrobial resistance genes were present in not only phenotypic resistant isolates of enterococci but also susceptible isolates. The investigation of antimicrobial resistance in enterococci during the cheesemaking process can be a source of valuable information for public health in the concept of "One Health".</description><subject>Amoxicillin</subject><subject>Ampicillin</subject><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Antimicrobial resistance</subject><subject>Bacteria</subject><subject>Cheese</subject><subject>Dairy industry</subject><subject>Dairy products</subject><subject>Drug resistance</subject><subject>enterococcal isolates</subject><subject>Erythromycin</subject><subject>Genes</subject><subject>Information processing</subject><subject>Lactic acid</subject><subject>Lactic acid bacteria</subject><subject>Levofloxacin</subject><subject>MALDI-TOF MS</subject><subject>Microorganisms</subject><subject>Milk</subject><subject>Nosocomial infection</subject><subject>Pasteurization</subject><subject>PCR</subject><subject>Penicillin</subject><subject>Plasmids</subject><subject>processing environment</subject><subject>Public health</subject><subject>Rifampin</subject><subject>silent genes</subject><subject>Vancomycin</subject><subject>Veterinary medicine</subject><issn>2075-1729</issn><issn>2075-1729</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdkU1P3DAQhi3UChBw67my1EsPLPjb8XG1ghYJCVTRszVxJqy3SUzt5NB_X8NShOqL7ZlnXo_nJeQTZxdSOnY5xB65YpY1jh2QY8GsXnEr3Id35yNyVsqO1WU0N406JEe11Eij5DHBuxCWnHEKSFNPr6YZcwophEjjROct0vt6x1Kes-s8xwITDHSzRSw4wq84PVKYOvqwxZjpeprjGENObazQDyyxzFClT8nHHoaCZ6_7Cfl5ffWw-b66vft2s1nfroLkbF6hUhZ6zrBtQOtOKts5BWB423FlZc1qUNiik8Ypx5GjAM0AsJMgDFh5Qm72ul2CnX_KcYT8xyeI_iWQ8qOH-ocwoA9WBiGUEYa1KgjZWN0JrM8Da7FXump93Ws95fR7wTL7MZaAwwATpqV4yRrNuWPOVfTLf-guLbnO6YVSTlYrmkqd76k6n1Iy9m8Ncuaf3fTv3az451fRpR2xe4P_eSf_AnrSmas</recordid><startdate>20240718</startdate><enddate>20240718</enddate><creator>Hanzelová, Zuzana</creator><creator>Dudriková, Eva</creator><creator>Lovayová, Viera</creator><creator>Výrostková, Jana</creator><creator>Regecová, Ivana</creator><creator>Zigo, František</creator><creator>Bartáková, Klára</creator><general>MDPI AG</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-0190-4346</orcidid><orcidid>https://orcid.org/0000-0002-3117-8915</orcidid><orcidid>https://orcid.org/0000-0002-2791-166X</orcidid><orcidid>https://orcid.org/0000-0002-7393-1960</orcidid></search><sort><creationdate>20240718</creationdate><title>Occurrence of Enterococci in the Process of Artisanal Cheesemaking and Their Antimicrobial Resistance</title><author>Hanzelová, Zuzana ; 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The species of the greatest clinical importance are
and
, which are common agents of nosocomial infections. However, enterococci also have important applications in the dairy industry, as they are used as non-starter lactic acid bacteria (NSLAB) in a variety of cheeses, especially artisanal cheeses. The aim of this study was to determine the presence of representatives from the
genus using PCR and MALDI-TOF MS methods on samples of raw milk, processing environment swabs, and cheese from four different artisanal dairy plants in Slovakia. Among the 136 isolates of enterococci, 9 species of genus
(
,
,
,
,
,
,
,
, and
) were identified and were tested for their antimicrobial resistance (AMR) to 8 antibiotics (amoxicillin, penicillin, ampicillin, erythromycin, levofloxacin, vancomycin, rifampicin, and tetracycline); most of them were resistant to rifampicin (35.3%), ampicillin (22.8%), and tetracycline (19.9%). A PCR analysis of
(4.41%) and
(14.71%) revealed that antimicrobial resistance genes were present in not only phenotypic resistant isolates of enterococci but also susceptible isolates. The investigation of antimicrobial resistance in enterococci during the cheesemaking process can be a source of valuable information for public health in the concept of "One Health".</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>39063643</pmid><doi>10.3390/life14070890</doi><orcidid>https://orcid.org/0000-0003-0190-4346</orcidid><orcidid>https://orcid.org/0000-0002-3117-8915</orcidid><orcidid>https://orcid.org/0000-0002-2791-166X</orcidid><orcidid>https://orcid.org/0000-0002-7393-1960</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Amoxicillin Ampicillin Antibiotics Antimicrobial agents Antimicrobial resistance Bacteria Cheese Dairy industry Dairy products Drug resistance enterococcal isolates Erythromycin Genes Information processing Lactic acid Lactic acid bacteria Levofloxacin MALDI-TOF MS Microorganisms Milk Nosocomial infection Pasteurization PCR Penicillin Plasmids processing environment Public health Rifampin silent genes Vancomycin Veterinary medicine |
| title | Occurrence of Enterococci in the Process of Artisanal Cheesemaking and Their Antimicrobial Resistance |
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