Genome-wide exploration of a pyroptosis-related gene module along with immune cell infiltration patterns in bronchopulmonary dysplasia

• Published in: Frontiers in genetics 2023-01, Vol.13, p.1074723-1074723
• Main Authors: Chen, Leiming, Shi, Chaofan, Zhou, Guoping, Yang, Xiaofeng, Xiong, Zhenqin, Ma, Xiaoxue, Zhu, Lan, Ma, Xuejiao, Mao, Yan, Hu, Yifang, Wang, Jimei, Tang, Xinfang, Bao, Yunlei, Ma, Yunxia, Luo, Fei, Wu, Chuyan, Jiang, Feng
• Format: Article
• Language: English
• Subjects: bioinformatics; bronchopulmonary dysplasia; diagnostic; Genetics; immune microenvironment; pyroptosis
• ISSN: 1664-8021; 1664-8021
• DOI: 10.3389/fgene.2022.1074723

Pyroptosis plays a crucial role in bronchopulmonary dysplasia (BPD) and is associated with various lung injury illnesses. However, the function of pyroptosis-related genes (PRGs) in BPD remains poorly understood. The gene expression omnibus (GEO) database was searched for information on genes associated with BPD. Twenty-five BPD-related DE-PRGs were identified, all of which were closely associated with pyroptosis regulation and immunological response. LASSO and SVM-RFE algorithms identified CHMP7, NLRC4, NLRP2, NLRP6, and NLRP9 among the 25 differentially expressed PRGs as marker genes with acceptable diagnostic capabilities. Using these five genes, we also generated a nomogram with excellent predictive power. Annotation enrichment analyses revealed that these five genes may be implicated in BPD and numerous BPD-related pathways. In addition, the ceRNA network showed an intricate regulatory link based on the marker genes. In addition, CIBERSORT-based studies revealed that alterations in the immunological microenvironment of BPD patients may be associated with the marker genes. We constructed a diagnostic nomogram and gave insight into the mechanism of BPD. Its diagnostic value for BPD must be evaluated in further research before it can be used in clinical practice.