The short neuropeptide F regulates appetitive but not aversive responsiveness in a social insect

• Published in: iScience 2022-01, Vol.25 (1), p.103619-103619, Article 103619
• Main Authors: Bestea, Louise, Paoli, Marco, Arrufat, Patrick, Ronsin, Brice, Carcaud, Julie, Sandoz, Jean-Christophe, Velarde, Rodrigo, Giurfa, Martin, de Brito Sanchez, Maria Gabriela
• Format: Article
• Language: English
• Subjects: Animal physiology; Behavioral neuroscience; Cognitive science; Entomology; Neuroscience
• ISSN: 2589-0042; 2589-0042
• DOI: 10.1016/j.isci.2021.103619

The neuropeptide F (NPF) and its short version (sNPF) mediate food- and stress-related responses in solitary insects. In the honeybee, a social insect where food collection and defensive responses are socially regulated, only sNPF has an identified receptor. Here we increased artificially sNPF levels in honeybee foragers and studied the consequences of this manipulation in various forms of appetitive and aversive responsiveness. Increasing sNPF in partially fed bees turned them into the equivalent of starved animals, enhancing both their food consumption and responsiveness to appetitive gustatory and olfactory stimuli. Neural activity in the olfactory circuits of fed animals was reduced and could be rescued by sNPF treatment to the level of starved bees. In contrast, sNPF had no effect on responsiveness to nociceptive stimuli. Our results thus identify sNPF as a key modulator of hunger and food-related responses in bees, which are at the core of their foraging activities. [Display omitted] •sNPF increases food consumption and appetitive responsiveness of honeybees•Feeding reduces neural activity in odor circuits; sNPF restores it to the starved level•sNPF has no effect on responsiveness to nociceptive stimuli in honeybees•sNPF is a key modulator of hunger and food-related responses in bees Entomology; Animal physiology; Behavioral neuroscience