Optimization of lipase production by Burkholderia sp. using response surface methodology

Response surface methodology (RSM) was employed to optimize the extracellular lipase production by Burkholderia sp. HL-10. Preliminary tests showed that olive oil, tryptone and Tween-80 exhibited significant effects on the lipase production. The optimum concentrations of these three components were...

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Bibliographic Details
Published in:International journal of molecular sciences 2012-11, Vol.13 (11), p.14889-14897
Main Authors: Lo, Chia-Feng, Yu, Chi-Yang, Kuan, I-Ching, Lee, Shiow-Ling
Format: Article
Language:English
Subjects:
Analysis of Variance
Burkholderia - drug effects
Burkholderia - metabolism
Burkholderia sp
Culture Media
Enzyme Activation - drug effects
Extracellular Space - metabolism
lipase
Lipase - biosynthesis
Olive Oil
Optimization
Peptones - pharmacology
Plant Oils - pharmacology
Polysorbates - pharmacology
response surface methodology
Variance analysis
Vegetable oils
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Summary:Response surface methodology (RSM) was employed to optimize the extracellular lipase production by Burkholderia sp. HL-10. Preliminary tests showed that olive oil, tryptone and Tween-80 exhibited significant effects on the lipase production. The optimum concentrations of these three components were determined using a faced-centered central composite design (FCCCD). The analysis of variance revealed that the established model was significant (p < 0.01). The optimized medium containing 0.65% olive oil (v/v), 2.42% tryptone (w/v) and 0.15% Tween-80 (v/v) resulted in a maximum activity of 122.3 U/mL, about three fold higher than that in basal medium. Approximately 99% of validity of the predicted value was achieved.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms131114889