The identification of high-performing antibodies for TDP-43 for use in Western Blot, immunoprecipitation and immunofluorescence [version 2; peer review: 2 approved]

TAR DNA-binding protein 43 (TDP-43) is a DNA/RNA binding protein playing a critical role in the regulation of transcription, splicing and RNA stability. Mutations in TARDBP leading to aggregation, are suspected to be a characteristic feature of various neurogenerative diseases. The lack of well-char...

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Bibliographic Details
Published in:F1000 research 2023, Vol.12, p.277-277
Main Authors: Worrall, Donovan, Ayoubi, Riham, Fotouhi, Maryam, Southern, Kathleen, McPherson, Peter S., Laflamme, Carl
Format: Article
Language:English
Subjects:
Amyotrophic lateral sclerosis
Antibodies
antibody characterization
antibody validation
Cell lines
Cells
Data Note
DNA-binding protein
eng
Gene regulation
Immunofluorescence
Immunoprecipitation
Invoices
Monoclonal antibodies
Mutation
Proteins
RNA-binding protein
TARDBP
TDP-43
Uniprot ID Q13148
Western Blot
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Summary:TAR DNA-binding protein 43 (TDP-43) is a DNA/RNA binding protein playing a critical role in the regulation of transcription, splicing and RNA stability. Mutations in TARDBP leading to aggregation, are suspected to be a characteristic feature of various neurogenerative diseases. The lack of well-characterized anti- TDP-43 antibodies acts as a barrier to establish reproducible TDP-43 research. In this study, we characterized eighteen TDP-43 commercial antibodies for Western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. We identified many well-performing antibodies and encourage readers to use this report as a guide to select the most appropriate antibody for their specific needs.
ISSN:2046-1402
2046-1402
DOI:10.12688/f1000research.131852.2