Loading…
Efficacy of Indigenous Bacteria in the Biodegradation of Hydrocarbons Isolated from Agricultural Soils in Huamachuco, Peru
Pollution from crude oil and its derivatives poses a serious threat to human health and ecosystems, with accidental spills causing substantial damage. Biodegradation, using microorganisms to break down these contaminants, presents a promising and cost-effective solution. Exploring and utilizing new...
Saved in:
| Published in: | Microorganisms (Basel) 2024-09, Vol.12 (9), p.1896 |
|---|---|
| Main Authors: | , , , , , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | |
|---|---|
| cites | |
| container_end_page | |
| container_issue | 9 |
| container_start_page | 1896 |
| container_title | Microorganisms (Basel) |
| container_volume | 12 |
| creator | Quiñones-Cerna, Claudio Castañeda-Aspajo, Alina Tirado-Gutierrez, Marycielo Salirrosas-Fernández, David Rodríguez-Soto, Juan Carlos Cruz-Monzón, José Alfredo Hurtado-Butrón, Fernando Ugarte-López, Wilmer Gutiérrez-Araujo, Mayra Quezada-Alvarez, Medardo Alberto Gálvez-Rivera, Julieta Alessandra Esparza-Mantilla, Mario |
| description | Pollution from crude oil and its derivatives poses a serious threat to human health and ecosystems, with accidental spills causing substantial damage. Biodegradation, using microorganisms to break down these contaminants, presents a promising and cost-effective solution. Exploring and utilizing new bacterial strains from underexplored habitats could improve remediation efforts at contaminated sites. This study aimed to evaluate the hydrocarbon biodegradation capacity of bacteria isolated from agricultural soils in Huamachuco, Peru. Soil samples from Oca crops were collected and bacteria were isolated. Biodegradation assays were conducted using diesel as the sole carbon source in the Bushnell Haas Mineral medium. Molecular characterization of the 16S rRNA gene identified four strains. Diesel biodegradation assays at 1% concentration were performed under agitation conditions at 150 rpm and 30 °C, and monitored on day 10 by measuring cellular biomass (OD
), with hydrocarbons analyzed by gas chromatography. The results showed
(PROM2) achieved the highest efficiency in removing total hydrocarbons (91.5 ± 0.7%). Additionally,
PROM3 and
ClyRoM5 also demonstrated high capacity in removing several individual hydrocarbons. Indigenous bacteria from uncontaminated agricultural soils present a high potential for hydrocarbon bioremediation, offering an ecological and effective solution for soil decontamination. |
| doi_str_mv | 10.3390/microorganisms12091896 |
| format | article |
| fullrecord | <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_c7b65f7bdec3467f9dae293adad84673</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A810763974</galeid><doaj_id>oai_doaj_org_article_c7b65f7bdec3467f9dae293adad84673</doaj_id><sourcerecordid>A810763974</sourcerecordid><originalsourceid>FETCH-LOGICAL-d411t-d7bd6b4bfe660729f40a99b11e8182054276a13fcef963cd282bfbe8d5e499a83</originalsourceid><addsrcrecordid>eNqNksFu1DAQhiMEolXpK1SWuHBgix0nTnxC26qwK1UCCThHE3u861ViFzuptDw9s92CWk74Ynv8-bf_mSmKC8EvpdT8w-hNijFtIPg8ZlFyLVqtXhSnJW_UolS8eflkfVKc57zjNLSQbS1eFydSS1o1_LT4deOcN2D2LDq2DtZvMMQ5syswEyYPzAc2bZFd-Whxk8DC5GM4wKu9TdFA6mPIbJ3jABNa5lIc2XKTvJmHaU4wsG_RD_kgs5phBLOdTXzPvmKa3xSvHAwZzx_ns-LHp5vv16vF7ZfP6-vl7cJWQkwL2_RW9VXvUJGZUruKg9a9ENiKtuR1VTYKhHQGnVbS2LIte9dja2ustIZWnhXro66NsOvukh8h7bsIvnsIUB47SJM3A3am6VXt6EE0slKN0xaw1JJc25b2krQ-HrXu5n5EazBM5PGZ6POT4LfdJt53QlSyko0mhXePCin-nDFP3eizwWGAgJT4Topatrym6v0HKqikXFUH9O0_6C7OKVBaHygla10eqMsjtQEy64OL9EcqPliklooBnaf4shXUOlI3FV24eGr3r88__SN_Aw4FzQw</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3110635927</pqid></control><display><type>article</type><title>Efficacy of Indigenous Bacteria in the Biodegradation of Hydrocarbons Isolated from Agricultural Soils in Huamachuco, Peru</title><source>Open Access: PubMed Central</source><source>Publicly Available Content (ProQuest)</source><creator>Quiñones-Cerna, Claudio ; Castañeda-Aspajo, Alina ; Tirado-Gutierrez, Marycielo ; Salirrosas-Fernández, David ; Rodríguez-Soto, Juan Carlos ; Cruz-Monzón, José Alfredo ; Hurtado-Butrón, Fernando ; Ugarte-López, Wilmer ; Gutiérrez-Araujo, Mayra ; Quezada-Alvarez, Medardo Alberto ; Gálvez-Rivera, Julieta Alessandra ; Esparza-Mantilla, Mario</creator><creatorcontrib>Quiñones-Cerna, Claudio ; Castañeda-Aspajo, Alina ; Tirado-Gutierrez, Marycielo ; Salirrosas-Fernández, David ; Rodríguez-Soto, Juan Carlos ; Cruz-Monzón, José Alfredo ; Hurtado-Butrón, Fernando ; Ugarte-López, Wilmer ; Gutiérrez-Araujo, Mayra ; Quezada-Alvarez, Medardo Alberto ; Gálvez-Rivera, Julieta Alessandra ; Esparza-Mantilla, Mario</creatorcontrib><description>Pollution from crude oil and its derivatives poses a serious threat to human health and ecosystems, with accidental spills causing substantial damage. Biodegradation, using microorganisms to break down these contaminants, presents a promising and cost-effective solution. Exploring and utilizing new bacterial strains from underexplored habitats could improve remediation efforts at contaminated sites. This study aimed to evaluate the hydrocarbon biodegradation capacity of bacteria isolated from agricultural soils in Huamachuco, Peru. Soil samples from Oca crops were collected and bacteria were isolated. Biodegradation assays were conducted using diesel as the sole carbon source in the Bushnell Haas Mineral medium. Molecular characterization of the 16S rRNA gene identified four strains. Diesel biodegradation assays at 1% concentration were performed under agitation conditions at 150 rpm and 30 °C, and monitored on day 10 by measuring cellular biomass (OD
), with hydrocarbons analyzed by gas chromatography. The results showed
(PROM2) achieved the highest efficiency in removing total hydrocarbons (91.5 ± 0.7%). Additionally,
PROM3 and
ClyRoM5 also demonstrated high capacity in removing several individual hydrocarbons. Indigenous bacteria from uncontaminated agricultural soils present a high potential for hydrocarbon bioremediation, offering an ecological and effective solution for soil decontamination.</description><identifier>ISSN: 2076-2607</identifier><identifier>EISSN: 2076-2607</identifier><identifier>DOI: 10.3390/microorganisms12091896</identifier><identifier>PMID: 39338570</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Accidental spills ; Acinetobacter ; agitation ; Agricultural ecosystems ; Agricultural land ; Bacteria ; Biodegradation ; biomass ; Bioremediation ; biotechnology ; carbon ; Carbon sources ; Chemical spills ; Contaminants ; cost effectiveness ; Crude oil ; Decontamination ; diesel ; Effectiveness ; Gas chromatography ; genes ; human health ; Hydrocarbons ; Microorganisms ; mitigation ; Oil pollution ; Oxidation ; Peru ; petroleum ; Pollution ; Polycyclic aromatic hydrocarbons ; Pseudomonas ; Pseudomonas protegens ; R&D ; Research & development ; Ribosomal DNA ; rRNA 16S ; soil ; Soil bacteria ; Soil contamination ; Soil disinfection ; Soil improvement ; Soil microbiology ; Soil microorganisms ; Soil pollution ; Soil remediation ; Soils ; Strains (organisms) ; Sulfur content</subject><ispartof>Microorganisms (Basel), 2024-09, Vol.12 (9), p.1896</ispartof><rights>COPYRIGHT 2024 MDPI AG</rights><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 by the authors. 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0002-9703-974X ; 0009-0004-4821-7046 ; 0000-0003-2640-9376 ; 0000-0003-0631-4900 ; 0000-0002-0215-5175 ; 0000-0002-9449-726X ; 0000-0003-3604-6054</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3110635927/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3110635927?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25744,27915,27916,37003,37004,44581,53782,53784,74887</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39338570$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Quiñones-Cerna, Claudio</creatorcontrib><creatorcontrib>Castañeda-Aspajo, Alina</creatorcontrib><creatorcontrib>Tirado-Gutierrez, Marycielo</creatorcontrib><creatorcontrib>Salirrosas-Fernández, David</creatorcontrib><creatorcontrib>Rodríguez-Soto, Juan Carlos</creatorcontrib><creatorcontrib>Cruz-Monzón, José Alfredo</creatorcontrib><creatorcontrib>Hurtado-Butrón, Fernando</creatorcontrib><creatorcontrib>Ugarte-López, Wilmer</creatorcontrib><creatorcontrib>Gutiérrez-Araujo, Mayra</creatorcontrib><creatorcontrib>Quezada-Alvarez, Medardo Alberto</creatorcontrib><creatorcontrib>Gálvez-Rivera, Julieta Alessandra</creatorcontrib><creatorcontrib>Esparza-Mantilla, Mario</creatorcontrib><title>Efficacy of Indigenous Bacteria in the Biodegradation of Hydrocarbons Isolated from Agricultural Soils in Huamachuco, Peru</title><title>Microorganisms (Basel)</title><addtitle>Microorganisms</addtitle><description>Pollution from crude oil and its derivatives poses a serious threat to human health and ecosystems, with accidental spills causing substantial damage. Biodegradation, using microorganisms to break down these contaminants, presents a promising and cost-effective solution. Exploring and utilizing new bacterial strains from underexplored habitats could improve remediation efforts at contaminated sites. This study aimed to evaluate the hydrocarbon biodegradation capacity of bacteria isolated from agricultural soils in Huamachuco, Peru. Soil samples from Oca crops were collected and bacteria were isolated. Biodegradation assays were conducted using diesel as the sole carbon source in the Bushnell Haas Mineral medium. Molecular characterization of the 16S rRNA gene identified four strains. Diesel biodegradation assays at 1% concentration were performed under agitation conditions at 150 rpm and 30 °C, and monitored on day 10 by measuring cellular biomass (OD
), with hydrocarbons analyzed by gas chromatography. The results showed
(PROM2) achieved the highest efficiency in removing total hydrocarbons (91.5 ± 0.7%). Additionally,
PROM3 and
ClyRoM5 also demonstrated high capacity in removing several individual hydrocarbons. Indigenous bacteria from uncontaminated agricultural soils present a high potential for hydrocarbon bioremediation, offering an ecological and effective solution for soil decontamination.</description><subject>Accidental spills</subject><subject>Acinetobacter</subject><subject>agitation</subject><subject>Agricultural ecosystems</subject><subject>Agricultural land</subject><subject>Bacteria</subject><subject>Biodegradation</subject><subject>biomass</subject><subject>Bioremediation</subject><subject>biotechnology</subject><subject>carbon</subject><subject>Carbon sources</subject><subject>Chemical spills</subject><subject>Contaminants</subject><subject>cost effectiveness</subject><subject>Crude oil</subject><subject>Decontamination</subject><subject>diesel</subject><subject>Effectiveness</subject><subject>Gas chromatography</subject><subject>genes</subject><subject>human health</subject><subject>Hydrocarbons</subject><subject>Microorganisms</subject><subject>mitigation</subject><subject>Oil pollution</subject><subject>Oxidation</subject><subject>Peru</subject><subject>petroleum</subject><subject>Pollution</subject><subject>Polycyclic aromatic hydrocarbons</subject><subject>Pseudomonas</subject><subject>Pseudomonas protegens</subject><subject>R&D</subject><subject>Research & development</subject><subject>Ribosomal DNA</subject><subject>rRNA 16S</subject><subject>soil</subject><subject>Soil bacteria</subject><subject>Soil contamination</subject><subject>Soil disinfection</subject><subject>Soil improvement</subject><subject>Soil microbiology</subject><subject>Soil microorganisms</subject><subject>Soil pollution</subject><subject>Soil remediation</subject><subject>Soils</subject><subject>Strains (organisms)</subject><subject>Sulfur content</subject><issn>2076-2607</issn><issn>2076-2607</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNksFu1DAQhiMEolXpK1SWuHBgix0nTnxC26qwK1UCCThHE3u861ViFzuptDw9s92CWk74Ynv8-bf_mSmKC8EvpdT8w-hNijFtIPg8ZlFyLVqtXhSnJW_UolS8eflkfVKc57zjNLSQbS1eFydSS1o1_LT4deOcN2D2LDq2DtZvMMQ5syswEyYPzAc2bZFd-Whxk8DC5GM4wKu9TdFA6mPIbJ3jABNa5lIc2XKTvJmHaU4wsG_RD_kgs5phBLOdTXzPvmKa3xSvHAwZzx_ns-LHp5vv16vF7ZfP6-vl7cJWQkwL2_RW9VXvUJGZUruKg9a9ENiKtuR1VTYKhHQGnVbS2LIte9dja2ustIZWnhXro66NsOvukh8h7bsIvnsIUB47SJM3A3am6VXt6EE0slKN0xaw1JJc25b2krQ-HrXu5n5EazBM5PGZ6POT4LfdJt53QlSyko0mhXePCin-nDFP3eizwWGAgJT4Topatrym6v0HKqikXFUH9O0_6C7OKVBaHygla10eqMsjtQEy64OL9EcqPliklooBnaf4shXUOlI3FV24eGr3r88__SN_Aw4FzQw</recordid><startdate>20240914</startdate><enddate>20240914</enddate><creator>Quiñones-Cerna, Claudio</creator><creator>Castañeda-Aspajo, Alina</creator><creator>Tirado-Gutierrez, Marycielo</creator><creator>Salirrosas-Fernández, David</creator><creator>Rodríguez-Soto, Juan Carlos</creator><creator>Cruz-Monzón, José Alfredo</creator><creator>Hurtado-Butrón, Fernando</creator><creator>Ugarte-López, Wilmer</creator><creator>Gutiérrez-Araujo, Mayra</creator><creator>Quezada-Alvarez, Medardo Alberto</creator><creator>Gálvez-Rivera, Julieta Alessandra</creator><creator>Esparza-Mantilla, Mario</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>7T7</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-9703-974X</orcidid><orcidid>https://orcid.org/0009-0004-4821-7046</orcidid><orcidid>https://orcid.org/0000-0003-2640-9376</orcidid><orcidid>https://orcid.org/0000-0003-0631-4900</orcidid><orcidid>https://orcid.org/0000-0002-0215-5175</orcidid><orcidid>https://orcid.org/0000-0002-9449-726X</orcidid><orcidid>https://orcid.org/0000-0003-3604-6054</orcidid></search><sort><creationdate>20240914</creationdate><title>Efficacy of Indigenous Bacteria in the Biodegradation of Hydrocarbons Isolated from Agricultural Soils in Huamachuco, Peru</title><author>Quiñones-Cerna, Claudio ; Castañeda-Aspajo, Alina ; Tirado-Gutierrez, Marycielo ; Salirrosas-Fernández, David ; Rodríguez-Soto, Juan Carlos ; Cruz-Monzón, José Alfredo ; Hurtado-Butrón, Fernando ; Ugarte-López, Wilmer ; Gutiérrez-Araujo, Mayra ; Quezada-Alvarez, Medardo Alberto ; Gálvez-Rivera, Julieta Alessandra ; Esparza-Mantilla, Mario</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-d411t-d7bd6b4bfe660729f40a99b11e8182054276a13fcef963cd282bfbe8d5e499a83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Accidental spills</topic><topic>Acinetobacter</topic><topic>agitation</topic><topic>Agricultural ecosystems</topic><topic>Agricultural land</topic><topic>Bacteria</topic><topic>Biodegradation</topic><topic>biomass</topic><topic>Bioremediation</topic><topic>biotechnology</topic><topic>carbon</topic><topic>Carbon sources</topic><topic>Chemical spills</topic><topic>Contaminants</topic><topic>cost effectiveness</topic><topic>Crude oil</topic><topic>Decontamination</topic><topic>diesel</topic><topic>Effectiveness</topic><topic>Gas chromatography</topic><topic>genes</topic><topic>human health</topic><topic>Hydrocarbons</topic><topic>Microorganisms</topic><topic>mitigation</topic><topic>Oil pollution</topic><topic>Oxidation</topic><topic>Peru</topic><topic>petroleum</topic><topic>Pollution</topic><topic>Polycyclic aromatic hydrocarbons</topic><topic>Pseudomonas</topic><topic>Pseudomonas protegens</topic><topic>R&D</topic><topic>Research & development</topic><topic>Ribosomal DNA</topic><topic>rRNA 16S</topic><topic>soil</topic><topic>Soil bacteria</topic><topic>Soil contamination</topic><topic>Soil disinfection</topic><topic>Soil improvement</topic><topic>Soil microbiology</topic><topic>Soil microorganisms</topic><topic>Soil pollution</topic><topic>Soil remediation</topic><topic>Soils</topic><topic>Strains (organisms)</topic><topic>Sulfur content</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Quiñones-Cerna, Claudio</creatorcontrib><creatorcontrib>Castañeda-Aspajo, Alina</creatorcontrib><creatorcontrib>Tirado-Gutierrez, Marycielo</creatorcontrib><creatorcontrib>Salirrosas-Fernández, David</creatorcontrib><creatorcontrib>Rodríguez-Soto, Juan Carlos</creatorcontrib><creatorcontrib>Cruz-Monzón, José Alfredo</creatorcontrib><creatorcontrib>Hurtado-Butrón, Fernando</creatorcontrib><creatorcontrib>Ugarte-López, Wilmer</creatorcontrib><creatorcontrib>Gutiérrez-Araujo, Mayra</creatorcontrib><creatorcontrib>Quezada-Alvarez, Medardo Alberto</creatorcontrib><creatorcontrib>Gálvez-Rivera, Julieta Alessandra</creatorcontrib><creatorcontrib>Esparza-Mantilla, Mario</creatorcontrib><collection>PubMed</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Agriculture & Environmental Science Database</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Biological Sciences</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Microorganisms (Basel)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Quiñones-Cerna, Claudio</au><au>Castañeda-Aspajo, Alina</au><au>Tirado-Gutierrez, Marycielo</au><au>Salirrosas-Fernández, David</au><au>Rodríguez-Soto, Juan Carlos</au><au>Cruz-Monzón, José Alfredo</au><au>Hurtado-Butrón, Fernando</au><au>Ugarte-López, Wilmer</au><au>Gutiérrez-Araujo, Mayra</au><au>Quezada-Alvarez, Medardo Alberto</au><au>Gálvez-Rivera, Julieta Alessandra</au><au>Esparza-Mantilla, Mario</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efficacy of Indigenous Bacteria in the Biodegradation of Hydrocarbons Isolated from Agricultural Soils in Huamachuco, Peru</atitle><jtitle>Microorganisms (Basel)</jtitle><addtitle>Microorganisms</addtitle><date>2024-09-14</date><risdate>2024</risdate><volume>12</volume><issue>9</issue><spage>1896</spage><pages>1896-</pages><issn>2076-2607</issn><eissn>2076-2607</eissn><abstract>Pollution from crude oil and its derivatives poses a serious threat to human health and ecosystems, with accidental spills causing substantial damage. Biodegradation, using microorganisms to break down these contaminants, presents a promising and cost-effective solution. Exploring and utilizing new bacterial strains from underexplored habitats could improve remediation efforts at contaminated sites. This study aimed to evaluate the hydrocarbon biodegradation capacity of bacteria isolated from agricultural soils in Huamachuco, Peru. Soil samples from Oca crops were collected and bacteria were isolated. Biodegradation assays were conducted using diesel as the sole carbon source in the Bushnell Haas Mineral medium. Molecular characterization of the 16S rRNA gene identified four strains. Diesel biodegradation assays at 1% concentration were performed under agitation conditions at 150 rpm and 30 °C, and monitored on day 10 by measuring cellular biomass (OD
), with hydrocarbons analyzed by gas chromatography. The results showed
(PROM2) achieved the highest efficiency in removing total hydrocarbons (91.5 ± 0.7%). Additionally,
PROM3 and
ClyRoM5 also demonstrated high capacity in removing several individual hydrocarbons. Indigenous bacteria from uncontaminated agricultural soils present a high potential for hydrocarbon bioremediation, offering an ecological and effective solution for soil decontamination.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>39338570</pmid><doi>10.3390/microorganisms12091896</doi><orcidid>https://orcid.org/0000-0002-9703-974X</orcidid><orcidid>https://orcid.org/0009-0004-4821-7046</orcidid><orcidid>https://orcid.org/0000-0003-2640-9376</orcidid><orcidid>https://orcid.org/0000-0003-0631-4900</orcidid><orcidid>https://orcid.org/0000-0002-0215-5175</orcidid><orcidid>https://orcid.org/0000-0002-9449-726X</orcidid><orcidid>https://orcid.org/0000-0003-3604-6054</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2076-2607 |
| ispartof | Microorganisms (Basel), 2024-09, Vol.12 (9), p.1896 |
| issn | 2076-2607 2076-2607 |
| language | eng |
| recordid | cdi_doaj_primary_oai_doaj_org_article_c7b65f7bdec3467f9dae293adad84673 |
| source | Open Access: PubMed Central; Publicly Available Content (ProQuest) |
| subjects | Accidental spills Acinetobacter agitation Agricultural ecosystems Agricultural land Bacteria Biodegradation biomass Bioremediation biotechnology carbon Carbon sources Chemical spills Contaminants cost effectiveness Crude oil Decontamination diesel Effectiveness Gas chromatography genes human health Hydrocarbons Microorganisms mitigation Oil pollution Oxidation Peru petroleum Pollution Polycyclic aromatic hydrocarbons Pseudomonas Pseudomonas protegens R&D Research & development Ribosomal DNA rRNA 16S soil Soil bacteria Soil contamination Soil disinfection Soil improvement Soil microbiology Soil microorganisms Soil pollution Soil remediation Soils Strains (organisms) Sulfur content |
| title | Efficacy of Indigenous Bacteria in the Biodegradation of Hydrocarbons Isolated from Agricultural Soils in Huamachuco, Peru |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T23%3A05%3A00IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Efficacy%20of%20Indigenous%20Bacteria%20in%20the%20Biodegradation%20of%20Hydrocarbons%20Isolated%20from%20Agricultural%20Soils%20in%20Huamachuco,%20Peru&rft.jtitle=Microorganisms%20(Basel)&rft.au=Qui%C3%B1ones-Cerna,%20Claudio&rft.date=2024-09-14&rft.volume=12&rft.issue=9&rft.spage=1896&rft.pages=1896-&rft.issn=2076-2607&rft.eissn=2076-2607&rft_id=info:doi/10.3390/microorganisms12091896&rft_dat=%3Cgale_doaj_%3EA810763974%3C/gale_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-d411t-d7bd6b4bfe660729f40a99b11e8182054276a13fcef963cd282bfbe8d5e499a83%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=3110635927&rft_id=info:pmid/39338570&rft_galeid=A810763974&rfr_iscdi=true |