Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride

Purpose. To optimize a rabbit dry eye model induced by topical instillation of benzalkonium chloride (BAC), reduce the days of instillation of the original model by increasing the concentration of BAC from 0.1% to 0.2%. Materials and Methods. An experimental, prospective, and randomized study was pe...

Full description

Saved in:
Bibliographic Details
Published in:Journal of ophthalmology 2020, Vol.2020 (2020), p.1-10
Main Authors: Carracedo, Gonzalo, Crooke, Almudena, Huete-Toral, Fernando, Pérez de Lara, María Jesús, Pintor, Jesús, Carpena-Torres, Carlos, Pastrana, Cristina
Format: Article
Language:English
Subjects:
Analysis
Animals
Cornea
Glaucoma
Inflammation
Povidone
Rabbits
Surface active agents
Ulcers
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Purpose. To optimize a rabbit dry eye model induced by topical instillation of benzalkonium chloride (BAC), reduce the days of instillation of the original model by increasing the concentration of BAC from 0.1% to 0.2%. Materials and Methods. An experimental, prospective, and randomized study was performed on 10 male New Zealand white rabbits, divided into two groups, considering both eyes: 5 rabbits as control (n = 10) and 5 rabbits with 0.2% BAC treatment (n = 10). Saline solution (control) and 0.2% BAC were instilled for 5 consecutive days, twice daily. Tear secretion with and without anesthesia, tear breakup time, tear osmolarity, corneal staining, conjunctival hyperemia, density of goblet cells, height of mucin cloud, and transcript levels of IL-6 were measured before and after the treatment. Results. After the instillation of 0.2% BAC for 5 consecutive days, there was a significant increase in tear secretion without anesthesia P
ISSN:2090-004X
2090-0058
DOI:10.1155/2020/7204951