Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride

Purpose. To optimize a rabbit dry eye model induced by topical instillation of benzalkonium chloride (BAC), reduce the days of instillation of the original model by increasing the concentration of BAC from 0.1% to 0.2%. Materials and Methods. An experimental, prospective, and randomized study was pe...

Full description

Saved in:
Bibliographic Details
Published in:Journal of ophthalmology 2020, Vol.2020 (2020), p.1-10
Main Authors: Carracedo, Gonzalo, Crooke, Almudena, Huete-Toral, Fernando, Pérez de Lara, María Jesús, Pintor, Jesús, Carpena-Torres, Carlos, Pastrana, Cristina
Format: Article
Language:English
Subjects:
Analysis
Animals
Cornea
Glaucoma
Inflammation
Povidone
Rabbits
Surface active agents
Ulcers
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c635t-e01ac8ce65c00eba8b03c1bb4e3b810786b142750c0220ae82c03ae1512c08743
cites cdi_FETCH-LOGICAL-c635t-e01ac8ce65c00eba8b03c1bb4e3b810786b142750c0220ae82c03ae1512c08743
container_end_page 10
container_issue 2020
container_start_page 1
container_title Journal of ophthalmology
container_volume 2020
creator Carracedo, Gonzalo
Crooke, Almudena
Huete-Toral, Fernando
Pérez de Lara, María Jesús
Pintor, Jesús
Carpena-Torres, Carlos
Pastrana, Cristina
description Purpose. To optimize a rabbit dry eye model induced by topical instillation of benzalkonium chloride (BAC), reduce the days of instillation of the original model by increasing the concentration of BAC from 0.1% to 0.2%. Materials and Methods. An experimental, prospective, and randomized study was performed on 10 male New Zealand white rabbits, divided into two groups, considering both eyes: 5 rabbits as control (n = 10) and 5 rabbits with 0.2% BAC treatment (n = 10). Saline solution (control) and 0.2% BAC were instilled for 5 consecutive days, twice daily. Tear secretion with and without anesthesia, tear breakup time, tear osmolarity, corneal staining, conjunctival hyperemia, density of goblet cells, height of mucin cloud, and transcript levels of IL-6 were measured before and after the treatment. Results. After the instillation of 0.2% BAC for 5 consecutive days, there was a significant increase in tear secretion without anesthesia P
doi_str_mv 10.1155/2020/7204951
format article
fullrecord <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_c7b88590b0ca4deaa6b3e0d35cffb373</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A632427100</galeid><doaj_id>oai_doaj_org_article_c7b88590b0ca4deaa6b3e0d35cffb373</doaj_id><sourcerecordid>A632427100</sourcerecordid><originalsourceid>FETCH-LOGICAL-c635t-e01ac8ce65c00eba8b03c1bb4e3b810786b142750c0220ae82c03ae1512c08743</originalsourceid><addsrcrecordid>eNqNktGL1DAQxoso3nHem89SEETQvZskTZO-COd66sLJgZzgW0jS6TZr26xNq-z99abuuncrPpg8ZJj85kvyZZLkKYEzQjg_p0DhXFDICk4eJMcUCpgBcPlwH2dfj5LTEFYQByMZ5_A4OWKU5znNxXGirteDa92tHpzvUl-lOv2sjXFD-q7fpJcbTD_5Ept00ZWjxTI1m_TGr53VUyoMrmn2lW-xu9XNN9-5sU3ndeN7V-KT5FGlm4Cnu_Uk-fL-8mb-cXZ1_WExv7ia2ZzxYYZAtJUWc24B0GhpgFliTIbMSAJC5oZkVHCwQClolNQC00g4iYEUGTtJFlvd0uuVWveu1f1Gee3U74Tvl0r3g7MNKiuMlLwAA1ZnJWqdG4ZQMm6ryjDBotabrdZ6NC2WFruh182B6OFO52q19D-UoEIAJVHg5U6g999HDINqXbAYverQj0HRjHBJC0qns57_ha782HfRqokCKJgg9I5a6vgA11U-nmsnUXWRMxqdiWikzv5BxVli66zvsHIxf1Dw4l5BjboZ6uCbcfrRcAi-3oK29yH0WO3NIKCmRlRTI6pdI0b82X0D9_CftovAqy1Qu67UP91_ymFksNJ3NJFFvCT7BVjZ68I</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2410093712</pqid></control><display><type>article</type><title>Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride</title><source>Publicly Available Content Database</source><source>Wiley Online Library (Open Access Collection)</source><source>PubMed Central</source><creator>Carracedo, Gonzalo ; Crooke, Almudena ; Huete-Toral, Fernando ; Pérez de Lara, María Jesús ; Pintor, Jesús ; Carpena-Torres, Carlos ; Pastrana, Cristina</creator><contributor>Figus, Michele ; Michele Figus</contributor><creatorcontrib>Carracedo, Gonzalo ; Crooke, Almudena ; Huete-Toral, Fernando ; Pérez de Lara, María Jesús ; Pintor, Jesús ; Carpena-Torres, Carlos ; Pastrana, Cristina ; Figus, Michele ; Michele Figus</creatorcontrib><description><![CDATA[Purpose. To optimize a rabbit dry eye model induced by topical instillation of benzalkonium chloride (BAC), reduce the days of instillation of the original model by increasing the concentration of BAC from 0.1% to 0.2%. Materials and Methods. An experimental, prospective, and randomized study was performed on 10 male New Zealand white rabbits, divided into two groups, considering both eyes: 5 rabbits as control (n = 10) and 5 rabbits with 0.2% BAC treatment (n = 10). Saline solution (control) and 0.2% BAC were instilled for 5 consecutive days, twice daily. Tear secretion with and without anesthesia, tear breakup time, tear osmolarity, corneal staining, conjunctival hyperemia, density of goblet cells, height of mucin cloud, and transcript levels of IL-6 were measured before and after the treatment. Results. After the instillation of 0.2% BAC for 5 consecutive days, there was a significant increase in tear secretion without anesthesia P<0.001, corneal staining P<0.001, conjunctival hyperemia P<0.001, and levels of IL-6 mRNA P=0.005 compared to the control group. Conversely, there was a decrease in tear secretion with anesthesia P<0.001, tear breakup time P=0.007, tear osmolarity P<0.001, density of goblet cells P<0.001, and height of mucin cloud P<0.001. Conclusions. The topical instillation of 0.2% BAC for 5 consecutive days, twice daily, was a proper procedure to induce a rabbit dry eye model, reducing the number of days of instillation compared to the original model (14 days).]]></description><identifier>ISSN: 2090-004X</identifier><identifier>EISSN: 2090-0058</identifier><identifier>DOI: 10.1155/2020/7204951</identifier><identifier>PMID: 32566267</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Analysis ; Animals ; Cornea ; Glaucoma ; Inflammation ; Povidone ; Rabbits ; Surface active agents ; Ulcers</subject><ispartof>Journal of ophthalmology, 2020, Vol.2020 (2020), p.1-10</ispartof><rights>Copyright © 2020 Carlos Carpena-Torres et al.</rights><rights>COPYRIGHT 2020 John Wiley &amp; Sons, Inc.</rights><rights>Copyright © 2020 Carlos Carpena-Torres et al. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright © 2020 Carlos Carpena-Torres et al. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c635t-e01ac8ce65c00eba8b03c1bb4e3b810786b142750c0220ae82c03ae1512c08743</citedby><cites>FETCH-LOGICAL-c635t-e01ac8ce65c00eba8b03c1bb4e3b810786b142750c0220ae82c03ae1512c08743</cites><orcidid>0000-0003-0054-1731 ; 0000-0001-9077-3557</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2410093712/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2410093712?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,4024,25753,27923,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32566267$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Figus, Michele</contributor><contributor>Michele Figus</contributor><creatorcontrib>Carracedo, Gonzalo</creatorcontrib><creatorcontrib>Crooke, Almudena</creatorcontrib><creatorcontrib>Huete-Toral, Fernando</creatorcontrib><creatorcontrib>Pérez de Lara, María Jesús</creatorcontrib><creatorcontrib>Pintor, Jesús</creatorcontrib><creatorcontrib>Carpena-Torres, Carlos</creatorcontrib><creatorcontrib>Pastrana, Cristina</creatorcontrib><title>Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride</title><title>Journal of ophthalmology</title><addtitle>J Ophthalmol</addtitle><description><![CDATA[Purpose. To optimize a rabbit dry eye model induced by topical instillation of benzalkonium chloride (BAC), reduce the days of instillation of the original model by increasing the concentration of BAC from 0.1% to 0.2%. Materials and Methods. An experimental, prospective, and randomized study was performed on 10 male New Zealand white rabbits, divided into two groups, considering both eyes: 5 rabbits as control (n = 10) and 5 rabbits with 0.2% BAC treatment (n = 10). Saline solution (control) and 0.2% BAC were instilled for 5 consecutive days, twice daily. Tear secretion with and without anesthesia, tear breakup time, tear osmolarity, corneal staining, conjunctival hyperemia, density of goblet cells, height of mucin cloud, and transcript levels of IL-6 were measured before and after the treatment. Results. After the instillation of 0.2% BAC for 5 consecutive days, there was a significant increase in tear secretion without anesthesia P<0.001, corneal staining P<0.001, conjunctival hyperemia P<0.001, and levels of IL-6 mRNA P=0.005 compared to the control group. Conversely, there was a decrease in tear secretion with anesthesia P<0.001, tear breakup time P=0.007, tear osmolarity P<0.001, density of goblet cells P<0.001, and height of mucin cloud P<0.001. Conclusions. The topical instillation of 0.2% BAC for 5 consecutive days, twice daily, was a proper procedure to induce a rabbit dry eye model, reducing the number of days of instillation compared to the original model (14 days).]]></description><subject>Analysis</subject><subject>Animals</subject><subject>Cornea</subject><subject>Glaucoma</subject><subject>Inflammation</subject><subject>Povidone</subject><subject>Rabbits</subject><subject>Surface active agents</subject><subject>Ulcers</subject><issn>2090-004X</issn><issn>2090-0058</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNktGL1DAQxoso3nHem89SEETQvZskTZO-COd66sLJgZzgW0jS6TZr26xNq-z99abuuncrPpg8ZJj85kvyZZLkKYEzQjg_p0DhXFDICk4eJMcUCpgBcPlwH2dfj5LTEFYQByMZ5_A4OWKU5znNxXGirteDa92tHpzvUl-lOv2sjXFD-q7fpJcbTD_5Ept00ZWjxTI1m_TGr53VUyoMrmn2lW-xu9XNN9-5sU3ndeN7V-KT5FGlm4Cnu_Uk-fL-8mb-cXZ1_WExv7ia2ZzxYYZAtJUWc24B0GhpgFliTIbMSAJC5oZkVHCwQClolNQC00g4iYEUGTtJFlvd0uuVWveu1f1Gee3U74Tvl0r3g7MNKiuMlLwAA1ZnJWqdG4ZQMm6ryjDBotabrdZ6NC2WFruh182B6OFO52q19D-UoEIAJVHg5U6g999HDINqXbAYverQj0HRjHBJC0qns57_ha782HfRqokCKJgg9I5a6vgA11U-nmsnUXWRMxqdiWikzv5BxVli66zvsHIxf1Dw4l5BjboZ6uCbcfrRcAi-3oK29yH0WO3NIKCmRlRTI6pdI0b82X0D9_CftovAqy1Qu67UP91_ymFksNJ3NJFFvCT7BVjZ68I</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>Carracedo, Gonzalo</creator><creator>Crooke, Almudena</creator><creator>Huete-Toral, Fernando</creator><creator>Pérez de Lara, María Jesús</creator><creator>Pintor, Jesús</creator><creator>Carpena-Torres, Carlos</creator><creator>Pastrana, Cristina</creator><general>Hindawi Publishing Corporation</general><general>Hindawi</general><general>John Wiley &amp; Sons, Inc</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-0054-1731</orcidid><orcidid>https://orcid.org/0000-0001-9077-3557</orcidid></search><sort><creationdate>2020</creationdate><title>Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride</title><author>Carracedo, Gonzalo ; Crooke, Almudena ; Huete-Toral, Fernando ; Pérez de Lara, María Jesús ; Pintor, Jesús ; Carpena-Torres, Carlos ; Pastrana, Cristina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c635t-e01ac8ce65c00eba8b03c1bb4e3b810786b142750c0220ae82c03ae1512c08743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Cornea</topic><topic>Glaucoma</topic><topic>Inflammation</topic><topic>Povidone</topic><topic>Rabbits</topic><topic>Surface active agents</topic><topic>Ulcers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carracedo, Gonzalo</creatorcontrib><creatorcontrib>Crooke, Almudena</creatorcontrib><creatorcontrib>Huete-Toral, Fernando</creatorcontrib><creatorcontrib>Pérez de Lara, María Jesús</creatorcontrib><creatorcontrib>Pintor, Jesús</creatorcontrib><creatorcontrib>Carpena-Torres, Carlos</creatorcontrib><creatorcontrib>Pastrana, Cristina</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access Journals</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Journal of ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carracedo, Gonzalo</au><au>Crooke, Almudena</au><au>Huete-Toral, Fernando</au><au>Pérez de Lara, María Jesús</au><au>Pintor, Jesús</au><au>Carpena-Torres, Carlos</au><au>Pastrana, Cristina</au><au>Figus, Michele</au><au>Michele Figus</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride</atitle><jtitle>Journal of ophthalmology</jtitle><addtitle>J Ophthalmol</addtitle><date>2020</date><risdate>2020</risdate><volume>2020</volume><issue>2020</issue><spage>1</spage><epage>10</epage><pages>1-10</pages><issn>2090-004X</issn><eissn>2090-0058</eissn><abstract><![CDATA[Purpose. To optimize a rabbit dry eye model induced by topical instillation of benzalkonium chloride (BAC), reduce the days of instillation of the original model by increasing the concentration of BAC from 0.1% to 0.2%. Materials and Methods. An experimental, prospective, and randomized study was performed on 10 male New Zealand white rabbits, divided into two groups, considering both eyes: 5 rabbits as control (n = 10) and 5 rabbits with 0.2% BAC treatment (n = 10). Saline solution (control) and 0.2% BAC were instilled for 5 consecutive days, twice daily. Tear secretion with and without anesthesia, tear breakup time, tear osmolarity, corneal staining, conjunctival hyperemia, density of goblet cells, height of mucin cloud, and transcript levels of IL-6 were measured before and after the treatment. Results. After the instillation of 0.2% BAC for 5 consecutive days, there was a significant increase in tear secretion without anesthesia P<0.001, corneal staining P<0.001, conjunctival hyperemia P<0.001, and levels of IL-6 mRNA P=0.005 compared to the control group. Conversely, there was a decrease in tear secretion with anesthesia P<0.001, tear breakup time P=0.007, tear osmolarity P<0.001, density of goblet cells P<0.001, and height of mucin cloud P<0.001. Conclusions. The topical instillation of 0.2% BAC for 5 consecutive days, twice daily, was a proper procedure to induce a rabbit dry eye model, reducing the number of days of instillation compared to the original model (14 days).]]></abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>32566267</pmid><doi>10.1155/2020/7204951</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-0054-1731</orcidid><orcidid>https://orcid.org/0000-0001-9077-3557</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2090-004X
ispartof Journal of ophthalmology, 2020, Vol.2020 (2020), p.1-10
issn 2090-004X
2090-0058
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_c7b88590b0ca4deaa6b3e0d35cffb373
source Publicly Available Content Database; Wiley Online Library (Open Access Collection); PubMed Central
subjects Analysis
Animals
Cornea
Glaucoma
Inflammation
Povidone
Rabbits
Surface active agents
Ulcers
title Optimization of a Rabbit Dry Eye Model Induced by Topical Instillation of Benzalkonium Chloride
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-20T10%3A56%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Optimization%20of%20a%20Rabbit%20Dry%20Eye%20Model%20Induced%20by%20Topical%20Instillation%20of%20Benzalkonium%20Chloride&rft.jtitle=Journal%20of%20ophthalmology&rft.au=Carracedo,%20Gonzalo&rft.date=2020&rft.volume=2020&rft.issue=2020&rft.spage=1&rft.epage=10&rft.pages=1-10&rft.issn=2090-004X&rft.eissn=2090-0058&rft_id=info:doi/10.1155/2020/7204951&rft_dat=%3Cgale_doaj_%3EA632427100%3C/gale_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c635t-e01ac8ce65c00eba8b03c1bb4e3b810786b142750c0220ae82c03ae1512c08743%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2410093712&rft_id=info:pmid/32566267&rft_galeid=A632427100&rfr_iscdi=true