Pathology and immunohistochemistry study of Newcastle disease field case in chicken in Indonesia

The aim of the study was to examine pathology and the distribution pattern of Newcastle disease virus (NDV) in internal organs of chickens from a field case using immunohistochemical staining. 10 groups of broiler, layer, and domestic chicken were collected from necropsy room Division of Pathology,...

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Bibliographic Details
Published in:Veterinary World 2017-09, Vol.10 (9), p.1066-1071
Main Authors: Etriwati, Ratih, Dewi, Handharyani, Ekowati, Setiyaningsih, Surachmi
Format: Article
Language:English
Subjects:
Animal vaccines
Atrophy
broiler
Bursa of Fabricius
Cecum
Chickens
domestic chicken
Duodenum
Encephalitis
Enteritis
Epidemiology
Heart diseases
Histopathology
Immunohistochemistry
layer
Lungs
Medical research
Myocarditis
Necropsy
Nephritis
Newcastle disease
Organs
Pancreatitis
Pathology
Pericarditis
Perihepatitis
Polymerase chain reaction
Poultry
Proventriculitis
Reverse transcription
Tonsil
Trachea
Tracheitis
Viruses
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Summary:The aim of the study was to examine pathology and the distribution pattern of Newcastle disease virus (NDV) in internal organs of chickens from a field case using immunohistochemical staining. 10 groups of broiler, layer, and domestic chicken were collected from necropsy room Division of Pathology, Bogor Agricultural University. These chickens were originated from West Java and collected based on pathologist diagnosis as suspect of Newcastle disease (ND). They were subsequently confirmed positive of ND with real-time-reverse transcription polymerase chain reaction assay. The respiratory, circulatory, digestive, lymphoreticular and central nervous systems were collected for histopathology examination. The gross pathology and histopathology changes were tracheitis, pneumonia, pericarditis, myocarditis, catarrhal proventriculitis, catarrhal enteritis, typhlitis, perihepatitis, pancreatitis, nephritis interstitial, splenitis, atrophy of Bursa Fabricius, and encephalitis. The distribution pattern of NDV in internal organs of chickens from a field case in this study is similar with a previous reported pattern in systemic cases of the internal chicken organs. High intensity of immunohistochemistry stain result was detected in trachea, lung, proventriculus, duodenum, cecal tonsil, kidney, and brain.
ISSN:0972-8988
2231-0916
DOI:10.14202/vetworld.2017.1066-1071