Mucus Microrheology Measured on Human Bronchial Epithelium Culture

We describe an original method to measure mucus microrheology on human bronchial epithelium culture using optical tweezers. We probed rheology on the whole thickness of mucus above the epithelium and showed that mucus gradually varies in rheological response, from an elastic behavior close to the ep...

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Bibliographic Details
Published in:Frontiers in physics 2019-02, Vol.7
Main Authors: Jory, Myriam, Bellouma, Karim, Blanc, Christophe, Casanellas, Laura, Petit, Aurélie, Reynaud, Paul, Vernisse, Charlotte, Vachier, Isabelle, Bourdin, Arnaud, Massiera, Gladys
Format: Article
Language:English
Subjects:
Biological Physics
Condensed Matter
human bronchial epithelium (HBE)
microrheology
mucins
mucus
optical tweezers (OT)
Physics
Soft Condensed Matter
viscoelasticity
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Summary:We describe an original method to measure mucus microrheology on human bronchial epithelium culture using optical tweezers. We probed rheology on the whole thickness of mucus above the epithelium and showed that mucus gradually varies in rheological response, from an elastic behavior close to the epithelium to a viscous one far away. Microrheology was also performed on mucus collected on the culture, on ex vivo mucus collected by bronchoscopy, and on another epithelium model. Differences are discussed and are related to mucus heterogeneity, adhesiveness and collection method.
ISSN:2296-424X
2296-424X
DOI:10.3389/fphy.2019.00019